Neogen® Molecular Detection System™ Approvals
Certifications, Recognitions, and Validations
Third-party validations confirm that our method has proven to be reliable and repeatable, and that it performs equal to or better than the reference method to which it was compared.
- U.S. Department of Agriculture Food Safety and Inspection Service (FSIS): Selected Neogen's Molecular Detection System 2 assays as the primary detection method for Salmonella, Listeria monocytogenes, Listeria spp. and Salmonella Enteritidis / Salmonella Typhimurium.
- U.S. Food & Drug Administration: Designated the Neogen Molecular Detection – Assay 2 Listeria as an equivalent testing methodology.
Understanding the Different Types of Validations
NF Validation by AFNOR Certification
A European validation scheme aligned with ISO 16140‑2 and ISO 16140‑6 protocols, recognized under EU Regulation (EC) 2073/2005 for alternative microbiological methods.
MicroVal
An international certification system validating alternative microbiological methods against ISO 16140 standards to confirm equivalence to reference methods.
AOAC Official Methods of Analysis℠ (OMA)
This AOAC method undergoes rigorous scientific review to ensure that performance requirements are met. Validated methods in this program are official methods and are recognized in the US Code of Federal Regulations.
AOAC Performance Tested Methods℠ (PTM)
Certified and validated methods under this program are recognized as alternative methods that have met performance requirements for their intended use.
International Recognition for Neogen® Molecular Detection Assays
AFNOR NF Validation following ISO 16140-2
| Product Description | Catalog Number | Certificate Number |
 MDA 2 Cronobacter | MDA2CRO96 | 3M 01/20-03/18 |
 MDA 2 E. coli O157 (including H7) | MDA2ECO96 | 3M 01/18-05/17 |
 MDA 2 Listeria | MDA2LIS96 | 3M 01/14-05/16 |
 MDA 2 Listeria monocytogenes | MDA2LMO96 | 3M 01/15-09/16 |
 MDA 2 Salmonella | MDA2SAL96 | 3M 01/16-11/16 |
AOAC® INTERNATIONAL Official Method of Analysis℠
| Product Description | Catalog Number | OMA Number |
| MDA 2 Cronobacter | MDA2CRO96 | 2018.01 |
| MDA 2 E. coli O157 (including H7) | MDA2ECO96 | 2017.01 |
| MDA 2 Listeria | MDA2LIS96 | 2016.07 |
| MDA 2 Listeria monocytogenes | MDA2LMO96 | 2016.08 |
| MDA 2 Salmonella | MDA2SAL96 | 2016.01 |
AOAC Research Institute Performance Tested Method℠
| Product Description | Catalog Number | PTM Number |
 MDA Campylobacter | MDA2ECO96 | 111803 |
| MDA 2 Cronobacter | MDA2CRO96 | 101703 |
 MDA Listeria Right Now | MDALRN96 | 042604 |
| MDA 2 Listeria | MDA2LIS96 | 111501 |
| MDA 2 Listeria monocytogenes | MDA2LMO96 | 081501 |
| MDA 2 Salmonella | MDA2SAL96 | 091501 |
 MDA 2 STEC Gene Screen (stx and eae) | MDA2STXEAE48 | 071902 |
 MDA 2 STEC Gene Screen (stx) | MDA2STX96 | 071903 |
National Recognition for Neogen® Molecular Detection Assays
MAPA (Ministry of Agriculture and Livestock) - Brazil
| Product Description | Catalog Number | Official Letter Number |
| MDA2 - Salmonella | MDA2SAL96 | Nº 11/2019/CDL/CGAL/SDA/MAPA |
| MDA2 - Listeria monocytogenes | MDA2LMO96 | Nº 9/2019/CDL/CGAL/SDA/MAPA |
 MDA2 - Salmonella Enteritidis / Salmonella Typhimurium | MDA2SEST48 | Nº 3/2026/DIDEL/COSLAB/CGAL/DTEC/SDA/MAPA |
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