Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans.
Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables.
Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol. After the sample is incubated for five hours in Modified Buffered Peptone Water with Pyruvate, Cefsulodin, Acriflavin and Vancomycin are added, followed by an additional incubation period. These antimicrobic supplements effectively suppress normal flora, while allowing the growth of viable O157:H7 cells (including STEC) and is capable of detecting <1 cfu g in foods. improved recovery of pathogenic>E. coli from difficult commodities was achieved with Modified Buffered Peptone Water with Pyruvate enrichment.
