Neogen® Fraser Broth

SKU N°  700003097  |  Numéro de catalogue  NCM0050A

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Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

Spécifications
MarqueNeogen® Culture Media
AnalyteListeria spp.
PlateformeMilieux de culture déshydratés
Masse500 grammes
Grammes par litre55,00
Litres par 500 g9,00
Dimensions du paquet4,60 pouce x 2,90 pouce x 8,60 pouce
Poids du paquet1,35 Livres

Informations supplémentaires

Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

Documents

Pour accéder au certificat d’analyse (CA), veuillez utiliser notre Recherche CA.

Technologie

Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin also. Enzymatic digest of animal tissues, casein, meat extract and yeast extract provide the essential vitamins, minerals, amino acids, nitrogen and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization. According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO) and then sub-cultured onto Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.

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