Megazyme® Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

SKU N°  700004221  |  Numéro de catalogue  E-HKGDH

QTÉ:
Quantité minimale requise : 1
Pour cet article, la quantité commandée doit être un multiple d’une boîte. Quantité par boîte : 1

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and analytical testing applications.

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Spécifications
MarqueMegazyme
Numéro CE

Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49

Conditions de stockage

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Exemples d'applicationsApplications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
FormulationIn 3.2 M ammonium sulphate
Forme physiqueSuspension
Numero CAS

Hexokinase: 9001-51-8
G6P-DH: 9001-40-5

ConcentrationHexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Synonymes

Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

La sourceYeast
ExpressionHexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Spécificité

Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Définition de l'unité

Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

Températures optimales (°C)40
Dimensions du paquet0,80 pouce x 0,80 pouce x 1,90 pouce
Poids du paquet0,06 Livres

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