Megazyme® Galactose dehydrogenase - Galactose mutarotase
1 ml; galactose dehydrogenase (200 U/ml) / galactose mutarotase (4 mg/ml)
SKU N° 700004220 | Numéro de catalogue E-GALMUT
High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and analytical testing applications.
Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.
For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)
| Spécifications | ||
|---|---|---|
| Marque | Megazyme | |
| Numéro CE | Galactose dehydrogenase: 1.1.1.48 | |
| Conditions de stockage | Storage Temperature: 2-8°C Stability: > 1 year under recommended storage conditions. Check vial for details. | |
| Exemples d'applications | Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via d-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR). | |
| Formulation | In 3.2 M ammonium sulphate | |
| Forme physique | Suspension | |
| Numero CAS | Galactose dehydrogenase: 9028-54-0 | |
| Synonymes | Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase | |
| La source | Escherichia coli | |
| Expression | Recombinant from Escherichia coli | |
| Spécificité | Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). (1) α-D-Galactose ↔ β-D-Galactose. The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2). (2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+ | |
| Activité spécifique | Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25°C | |
| Définition de l'unité | One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute at pH 8.6, at 25°C. | |
| Températures optimales (°C) | 25 | |
| Dimensions du paquet | 0,70 pouce x 0,70 pouce x 1,60 pouce | |
| Poids du paquet | 0,04 Livres | |
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