Megazyme® Fructanase Mixture (powder)

20,000 Units

SKU N°  700004216  |  Numéro de catalogue  E-FRMXPD

AOAC OMA

QTÉ:
Quantité minimale requise : 1
Pour cet article, la quantité commandée doit être un multiple d’une boîte. Quantité par boîte : 1

High purity Fructanase Mixture (powder) for use in research, biochemical enzyme assays and analytical testing applications.

Components: exo-Inulinase 20,000 U (on kestose at 40°C), endo-inulinase 1000 U (on fructan at 40°C).

Fructanase, along with Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY), can also be utilised in the enzyme hydrolysis step for determining polydextrose a low molecular weight dietary fiber in food samples.

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan and Polydextrose.

Megazyme_Scheme-E-FRMXPD.png
Spécifications
MarqueMegazyme
Validations

For use with AOAC OMA 997.08, 2000.11, 2016.06

Numéro CE

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Conditions de stockage

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

FormulationSupplied as a lyophilised powder
Forme physiquePowder
Numero CAS

9001-57-4,
9025-67-6,
37288-56-5

Synonymes

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

La sourceAspergillus sp.
ExpressionFrom Aspergillus sp.
Spécificité

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Définition de l'unité

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Températures optimales (°C)40
Dimensions du paquet0,80 pouce x 0,80 pouce x 1,90 pouce
Poids du paquet0,03 Livres

Assistance