Reveal® for Pathogens
Neogen® Reveal® lateral flow tests deliver rapid, qualitative pathogen screening for Salmonella, Listeria, and E. coli O157:H7 in food and environmental samples. With validated methods, irradiated media for easy preparation, and rapid results, Reveal gives your team the speed to act and the confidence to trust every result.
Reveal® Lateral Flow Pathogen Screening for Food & Environmental Samples
Our Reveal tests offer flexible pathogen testing with accurate qualitative results using antibody-based lateral flow strips. The easy-to-use, easy-to-interpret lateral flow strips allow for microbial detection and offer scalability when it comes to sample throughput.
Reveal is a great fit for those looking for a quick and easy test to screen for pathogen presence.
Why Lateral Flow for Pathogen Screening?
Not every pathogen test needs to be molecular. Lateral flow immunoassays like Reveal offer a fast, cost-effective screening option that fits naturally into production-driven workflows — especially where speed, simplicity, and minimal equipment are priorities.
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Minimal hands-on time to prepare sample. Specific tests: Salmonella (Group A to E), Listeria spp. and E. coli O157:H7
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Irradiated media for easy preparation
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Simple visual interpretation of results.
Looking for Greater Sensitivity?
LAMP-powered pathogen detection built for speed, sensitivity, and streamlined workflows.


Reveal Pathogen Tests


Reveal® for E. coli O157:H7
AOAC Official Method for the detection of E. coli O157:H7 in selected foods — with flexible enrichment protocols delivering results in as little as 8 hours for 25 g samples.
- Validations: AOAC OMA 2000.13; AOAC OMA 2000.14
- In an AOAC Official Methods℠ study (2000.13), the Reveal for E. coli O157:H7 8-hour test system was found to be an effective procedure for the detection of E. coli O157:H7 in raw ground beef, raw beef cubes, and iceberg lettuce rinse.
- In a second AOAC Official Methods study (2000.14), the Reveal for E. coli O157:H7 20-hour test system was found to be an effective procedure for the detection of E. coli O157:H7 in raw ground beef, raw beef cubes, apple cider, iceberg lettuce rinse, and stainless steel environmental swabs.
- LOD: 1 cfu/analytical unit
- Test Time: 15 minutes (device read)
- Time to Result: 8–20 hours
- Enrichment Options:
- 8-hour test system: 8 hours (25 g) or 12 hours (375 g)
- 20-hour test system: 20 hours (25 g or 375 g)


Reveal® 2.0 for E. coli O157:H7
Designed for the detection and presumptive identification of E. coli O157:H7 and E. coli O157:NM in various foods — with results in as little as 12-20 hours.
- Validations: AOAC® PTM certified (Certificate No 011103) for detection of E. coli O157:H7 in raw beef trim and raw ground beef
- LOD: 1 cfu/analytical unit
- Test Time: 15 minutes (device read)
- Time to Result: 12–20 hours
- Enrichment: 12–20 hour protocols for 65 g and 375 g samples; irradiated media included


Reveal® 2.0 for Salmonella
Rapid recovery of Salmonella in food, animal feed, and environmental samples allowing detection and presumptive identification of the test organism, generally within 24 hours.
- Validations:
- AOAC PTM (Certificate No. 960801) — validated across 25+ food, feed, and environmental matrices. Detailed list of matrices.
- AFNOR NF Validation
- LOD: 1 cfu/analytical unit
- Test Time: 15 minutes (device read)
- Time to Result: 20–48 hours
- Enrichment: Optimized protocols for 25 g and 325 g samples; irradiated media included


Reveal® 2.0 for Listeria
Designed for the detection and presumptive identification of Listeria species in food and environmental samples — with results in 27–30 hours.
- Validations:
- AOAC PTM Certificate No 041101 for detection of Listeria spp.
- AFNOR NF Validation
- LOD: 1 cfu/analytical unit
- Test Time: 20 minutes (device read)
- Time to Result: 27-30 hours (one-step protocol)
- Enrichment: LESS medium (one-step) or Half Fraser Broth Plus / BLEB (48-hour protocol)

Pathogen Testing Resources & Insights
Browse expert articles covering detection strategies, regulatory considerations, and real-world applications.

Reveal International Validations
For a complete list of Certifications, Recognitions, and Validations, see the Reveal Approvals page.