Reveal® 3-D: Rapid Food Allergen Detection
Neogen led the way with the first commercially available rapid allergen test and continued to lead with the Reveal® lateral flow platform—pioneering on-site allergen testing and shaping modern allergen control programs.
Built on this legacy, Reveal® 3-D delivers trusted, actionable results in minutes.
Lateral flow testing solutions: Qualitative results in minutes
In industries where allergen control is critical, teams face common challenges—from verifying cleaning effectiveness and preventing cross-contact in shared facilities to screening high-risk raw materials and responding to allergen complaints. Neogen supports food manufacturing and handling operations with solutions designed to deliver clear, actionable results in minutes—not hours—combining proven sensitivity with easy interpretation.
Designed by experts who rely on these tools every day, Neogen lateral flow tests (qualitative immunochromatographic assays) simplify allergen detection for rapid, on-site monitoring. These easy-to-use devices enable screening for low levels of allergens across environmental swabs, clean-in-place (CIP) rinses, and validated ingredients and product matrices. With an intuitive format, teams can prepare, dip, and read—reducing training time and increasing efficiency.
Our portfolio extends beyond Reveal® 3-D, offering rapid allergen tests for a wide range of targets, including cashew, fish, and walnut. Explore the full list of Reveal 3-D and rapid tests below.
Reveal 3-D: Rapid screening as part of a complete allergen testing strategy.
Rapid Allergen Testing for Confident Food Safety Decisions
Reveal 3-D provides fast, on-site allergen testing with low limits of detection and validated performance across surfaces, rinse waters, ingredients, and finished products, helping teams take immediate action and determine when confirmatory quantitative testing is needed.
Fast results
Device time to result in 5-11 minutes
Backed by experts
A full list of Certifications, Recognitions, and Validations is available on the Reveal Approvals page.
Low limits of detection
Sensitivity, specificity, and consistency
Versatile applications
Surfaces, CIP rinse water, ingredients, and food products
Trusted science
Over 2,800 matrix feasibility verifications completed
Validated Methods. Defensible Results.
Reveal and Neogen lateral flow rapid allergen tests are built on recognized, validated methodologies trusted by QA teams, regulatory bodies, and customer auditors worldwide.
- AOAC® Performance Tested Methods℠ — Reveal 3-D for Gluten and Reveal 3-D for Peanut hold AOAC PTM certification (No. 122201, 111901)
- GFCO Accepted — Listed on the Gluten-Free Certification Organization's approved immunoassay list for gluten testing
From food testing to CIP rinse verification to environmental swab screening, Reveal gives you results that are defensible, not just fast.
Hook Line Assurance
Our innovative overload design means less false negative worries
Our hook line feature can alert you of a possible false negative due to high amounts of target protein in a sample. This feature can increase confidence in your allergen testing results.
- Positive result: Allergen detected in the sample
- Negative result: No allergen detected in the sample
- Overloaded result: If neither the hook line nor test line appears, the sample may contain too much allergen protein and should be diluted and re‑tested
Learn how Reveal 3‑D hook line assurance helps reduce the risk of false negatives—read our blog: Why Hook Lines Are a Must‑Have.
Reveal® 3-D Allergen Testing How-To Video: 4 Simple Steps
This how‑to video walks through how to run a Reveal 3‑D allergen test in just four simple steps—from sample preparation to result interpretation.
What you’ll learn:
- Proper sample preparation and test setup for accurate allergen detection
- Best practices for timing, handling, and interpreting results
Reveal 3-D: Rapid screening as part of a complete allergen testing strategy.
Reveal for Multi-Treenut
In addition to Reveal 3-D, Neogen offers a rapid lateral flow assay capable of detecting six different tree nuts in a single test.
What you’ll learn:
- Time to result: 6 allergens in 1 kit in 10 min
- Detects: Pecan, Walnut, Cashew, Pistachio, Almond, and Hazelnut
Discover Reveal Allergen Detection Solutions
Rapid allergen detection solutions for accurate food safety testing and compliance.
Educational Resources
Allergen Products List
Comprehensive testing options designed to help you identify and manage food allergens with confidence across your operation.
Environmental Monitoring for Food Allergens
An overview of how environmental monitoring can strengthen allergen management and help manufacturers proactively reduce risk.
Allergen Risk Assessment: When to Use Which Test Kits
Understand when allergen test kits are most effective—and how to use risk assessment to support informed testing decisions.
Allergen Testing Methods Explained: Lateral Flow Assay vs. ELISA
Learn the key differences between lateral flow assays and ELISA testing—and how to choose the right method for your allergen program.
Allergen Handbook
Developed in collaboration with the University of Nebraska’s Food Allergy Research & Resource Program (FARRP), this handbook helps food manufacturers build effective allergen control programs.
Validation & Verification Allergens Handbook
Confirm that your allergen cleaning procedures are effectively removing residues and reducing cross-contact risk. Gain guidance from initial validation through ongoing verification to ensure methods perform as intended.
2nd Edition Neogen Environmental Monitoring Handbook
Developed with Cornell University and global experts, the 2nd Edition of the Neogen® EM handbook features dedicated guidance on Environmental Monitoring for Allergens.