Reveal® Q+ MAX for Fumonisin

Assay Principle

Reveal Q+ MAX for Fumonisin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for fumonisin conjugated to colloidal gold particles. If fumonisin is present, it will be captured by the particle-antibody complex. The fumonisin-antibody-particle complex then is wicked onto a membrane, which contains a zone of fumonisin conjugated to a protein carrier. This zone captures any uncomplexed fumonisin antibody, allowing the particles to concentrate and form a visible line. As the level of fumonisin in a sample increases, free fumonisin will form a complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of fumonisin in the sample increases, the test line density decreases. Algorithms programmed into the Raptor and Raptor Solo^® readers convert these line densities into a quantitative result displayed in parts per million (ppm). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line always will form regardless of the presence of fumonisin, ensuring the strip is functioning properly.