Neogen® Thioglycollate Medium without Indicator

Thioglcyollate Medium w/o Indicator is used for the cultivation of anaerobic microorganisms in a laboratory setting. Thioglcyollate Medium w/o Indicator is not intended for use in the diagnosis of disease or other conditions in humans.

Quastel and Stephenson found the presence of small amounts of a compound containing an –SH group (cysteine, thioglycollic acid, and glutathione) permitted “aerobic” growth of Clostridium sporogenes. Falk, Bucca, and Simmons discovered the advantages of using small quantities of agar in detecting contaminants during sterility testing. Brewer demonstrated the value of a small amount of agar and a reducing substance in this medium.

Thioglycolate Medium w/o Indicator is used for cultivating and detecting microorganisms in normally sterile materials, especially those containing mercurial preservatives when the oxidation-reduction indicator is not present or required. Thioglycolate Medium w/o Indicator is the medium of choice for diagnostic testing, where lack of an indicator avoids possible toxicity to organisms.