Neogen® Fraser Broth
Nº de SKU  700003097  | Número de catálogo  NCM0050A
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Neogen® Fraser Broth

Nº de SKU  700003097  |  Número de catálogo  NCM0050A

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Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

Especificações
MarcaNeogen® Culture Media
Tipo de amostraListeria spp.
PlataformaMeio de cultura desidratado
Massa500 g
Gramas por litro55,00
Litros por 500 g9,00
Dimensões da embalagem4,60 polegadas x 2,90 polegadas x 8,60 polegadas
Peso da embalagem1,35 libra

Informações adicionais

Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

Documentos

Para acessar o Certificado de Análise (C de A), use nossa Pesquisa de C de A.

Neogen® Fraser Broth Product Instructions - English - US
Fraser Broth - English - US Fraser Broth - German - Belgium Fraser Broth - French - France/Monaco Fraser Broth - English - Ireland Fraser Broth - Bulgarian - Bulgaria Fraser Broth - Korean - Korea Fraser Broth - Finnish - Finland Fraser Broth - French - Luxembourg Fraser Broth - Romanian - Romania Fraser Broth - Italian - Italy Fraser Broth - English - Canada Fraser Broth - Icelandic - Iceland Fraser Broth - Slovak - Slovakia Fraser Broth - Hungarian - Hungary Fraser Broth - Estonian - Estonia Fraser Broth - Spanish - US Fraser Broth - Norwegian - Norway Fraser Broth - French - Switzerland Fraser Broth - Dutch - Netherlands Fraser Broth - Danish - Denmark Fraser Broth - Dutch - Belgium NCM0050_fr-CA_SDS_v.1.0.pdf Fraser Broth - English - Great Britain Fraser Broth - French - Belgium Fraser Broth - Croatian - Croatia Fraser Broth - Polish - Poland Fraser Broth - English - Malta Fraser Broth - Greek - Greece Fraser Broth - Portuguese - Portugal Fraser Broth - Italian - Switzerland Fraser Broth - Spanish - Spain Fraser Broth - German - Luxembourg Fraser Broth - Latvian - Latvia Fraser Broth - Swedish - Sweden Fraser Broth - Slovene - Slovenia Fraser Broth - German - Austria Fraser Broth - Lithuanian - Lithuania Fraser Broth - Maltese - Malta Fraser Broth - Czech - Czech Republic Fraser Broth - Greek - Cyprus Fraser Broth - German - Switzerland Fraser Broth - German - Germany/Liechtenstein

Tecnologia

Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin also. Enzymatic digest of animal tissues, casein, meat extract and yeast extract provide the essential vitamins, minerals, amino acids, nitrogen and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization. According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO) and then sub-cultured onto Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.