Megazyme® Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)
100/200 assays per kit
Nº de SKU 700004329 | Número de catálogo K-PULLG6
- High sensitivity
- Suitable for manual and auto-analyser formats
- No transglycosylation interference
- Very cost effective
- All reagents stable for > 1 year after preparation
- Very specific
- Simple format
- Standard included
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
| Especificações | ||
|---|---|---|
| Marca | Megazyme | |
| Tipo de amostra | Pululanase, Limite-dextrinase | |
| Plataforma | Espectrofotômetro | |
| Tipo de resultado | Absorção UV | |
| Aprovações | Novel method | |
| Limite de detecção | 0.18 U/mL for pullulanase preparations (50-fold dilution), 0.01 U/g for limit dextrinase in milled malt | |
| Condições de armazenamento | Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions | |
| Comprimento de onda | 400 nm | |
| Exemplos de aplicação | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. | |
| Resposta do sinal | Increase | |
| Tempo total do ensaio | Approx. 10 min (pullanase); Approx. 30 min (limit-dextrinase) | |
| Reprodutibilidade | ~ 3% | |
| Dimensões da embalagem | 10,00 polegadas x 5,00 polegadas x 2,00 polegadas | |
| Peso da embalagem | 0,50 libra | |
Documentos
Para acessar o Certificado de Análise (C de A), use nossa Pesquisa de C de A.