MiSeq Reagent Kit v3 (600-cycle)
Nº de SKU  11502  | Número de catálogo 
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MiSeq Reagent Kit v3 (600-cycle)

Nº de SKU  11502  |  Número de catálogo 

  • Max read length of 2 x 300 bp.
  • Our longest available read-length flow cell.
  • Common for de novo and amplicon sequencing.
  • 20–25 million read-pairs.
  • Estimated output of 13.2–15 Gb.
  • FASTQC report included with all prepared pool orders.
  • All prepared pools will be QC'd at Neogen. If the results indicate poor quality, we will contact you.
  • 4 week turnaround time.
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To order or for more information contact us.
To order or for more information contact us.

The Illumina MiSeq benchtop sequencer is ideal for de novo assembly of small genomes or amplicon sequencing projects. Illumina MiSeq Reagent Kits v3 contain improved chemistry to increase cluster density and read depth as well as improve quality (Q) scores. The Illumina MiSeq Reagent Kit v3 600 cycle kit enables a max read length of 2 x 300 bp, with approximately 20–25 million read-pairs. Each prepared pool order will include a FASTQC report for the sequencing run.

Especificações
MarcaIllumina
PlataformaSequenciamento
Informações sobre o envio50 uL of your prepared pool at a concentration of 5 nM, determined by fluorometry or qPCR, is required. (the u in uL should be the micro symbol). Please ship your 5 nM pool in a screw cap tube within a para-filmed 50 mL centrifuge tube at the requested volume on dry ice to Neogen Genomics, 4131 N 48th St., Lincoln, NE 68504.
Tempo de resposta4 Weeks
FASTQ and FASTQC delivery via AWS

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Q: What should the concentration of the prepared pools be for submission?

A: Prepared pools should be submitted to Neogen at a concentration of 5 nM, determined by fluorometry or qPCR.

Q: How much volume of the prepared pool is needed for sequencing?

A: MiSeq runs require 50 uL of the prepared pool. NovaSeq runs require 4 unique pools of 100 uL.

Q: Where and how should the prepared pools be shipped?

A: Please ship your 5 nM prepared pool in a screw cap tube within a para-filmed 50 mL centrifuge tube at the requested volume on dry ice to Neogen Genomics, 4131 N 48th St., Lincoln, NE 68504.

Q: How will sequencing and QC data be delivered?

A: Data will be delivered via Amazon Web Services (AWS).

Q: Will Neogen perform library pooling?

A: No. At this time, Neogen will not perform library pooling. Libraries must be pooled before submitting for sequencing.