Megazyme® Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

Nº de SKU  700004221  |  Número de catálogo  E-HKGDH

QTD:
Quantidade mínima necessária: 1
Este artículo debe pedirse en múltiplos de una sola caja. Cantidad por caja: 1

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and analytical testing applications.

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Especificações
MarcaMegazyme
Número CE

Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49

Condições de armazenamento

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Exemplos de aplicaçãoApplications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
FormulaçãoIn 3.2 M ammonium sulphate
Forma físicaSuspension
Número CAS

Hexokinase: 9001-51-8
G6P-DH: 9001-40-5

ConcentraçãoHexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Sinônimos

Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

FonteYeast
ExpressãoHexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Especificidade

Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Definição de Unidade

Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

Temperatura ótima (°C)40
Dimensões da embalagem0,80 polegadas x 0,80 polegadas x 1,90 polegadas
Peso da embalagem0,06 libra