Megazyme® β-Glucuronidase (Escherichia coli)

3.2.1.31

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

9001-45-0

β-D-glucuronoside glucuronosohydrolase; GUS

82600

Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.

~ 15,000 U/mg (37°C, pH 6.8 on phenolphthalein-β-D-glucuronide);
~ 50 U/mg (37°C, pH 7.5 on pNP-β-D-glucuronide)

30000 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37°C.
110 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris.HCl buffer (100 mM) pH 7.5 and 37°C., monitored at 410 nm.