Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)
Nº de SKU  700004214  | Número de catálogo  E-FRLQPU
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Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)

10 ml / 20,000 Units

Nº de SKU  700004214  |  Número de catálogo  E-FRLQPU

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Quantidade mínima necessária: 1
Este artículo debe pedirse en múltiplos de una sola caja. Cantidad por caja: 1
To order or for more information contact our food safety team
To order or for more information contact our food safety team

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Components: exo-Inulinase (2,000 U/mL on kestose, at 40°C) and endo-Inulinase (100 U/mL on inulin at 40°C).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

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Especificações
MarcaMegazyme
Número CE

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Condições de armazenamento

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

Exemplos de aplicaçãoApplications in carbohydrate research and in the food industry.
FormulaçãoIn 50% (v/v) glycerol plus 0.02% sodium azide
Forma físicaSolution
Número CAS

9001-57-4,
9025-67-6,
37288-56-5

Concentraçãoendo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 10,000 U
Sinônimos

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

FonteAspergillus niger
ExpressãoRecombinant from Aspergillus niger
Especificidade

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Definição de Unidade

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Temperatura ótima (°C)60
Dimensões da embalagem0,80 polegadas x 0,80 polegadas x 1,90 polegadas
Peso da embalagem0,06 libra