Neogen® Fraser Broth
SKU 编号  700003097  | 目录编号  NCM0050A
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Neogen® Fraser Broth

SKU 编号  700003097  |  目录编号  NCM0050A

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最低数量要求：1
This item must be ordered in multiples of one case. Quantity per case: 1
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Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

规格
品牌Neogen® Culture Media
分析物李斯特菌属
平台脱水培养基
质量500 g
克每升55.00
每 500 g 升数9.00
包装尺寸4.60 in x 2.90 in x 8.60 in
包装重量1.35 lb

其他信息

Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

文件

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科技

Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin also. Enzymatic digest of animal tissues, casein, meat extract and yeast extract provide the essential vitamins, minerals, amino acids, nitrogen and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization. According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO) and then sub-cultured onto Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.