Veratox® para alérgeno de mostaza
SKU No.  700002561  | Número de catálogo  8400
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Veratox® para alérgeno de mostaza

Veratox® for Mustard

SKU No.  700002561  |  Número de catálogo  8400

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Cantidad:
Cantidad mínima necesaria: 1
É necessário fazer o pedido do item em múltiplos de uma caixa. Quantidade por caixa: 1
To order or for more information contact us
To order or for more information contact us

El ensayo Veratox® para alérgeno de mostaza se utiliza para el análisis cuantitativo de proteínas de mostaza en productos alimenticios como especias, salsas, aderezos, carnes y enjuagues de limpieza-en-sitio.

Especificaciones
MarcaVeratox®
AnalitoMostaza
PlataformaELISA
Tipo de resultadoCuantitativo
Límite de detección

<1 ppm mustard

Tiempo de la prueba30,00
Advertencia de prop. 65
Warning
ADVERTENCIA: Este producto puede exponerlo a productos químicos que incluyen timerosal, conocido en el estado de California por causar defectos congénitos u otros daños a la función reproductora. Para obtener más información, visite www.P65Warnings.ca.gov.
Cantidad por paquete48 pruebas
Dimensiones del empaque10,50 pulgada x 8,00 pulgada x 2,75 pulgada
Peso del paquete0,90 libra
  1. 48 antibody-coated microwells (24 per pouch)
  2. 48 red-marked transfer wells (24 per pouch)
  3. 5 yellow-labeled bottles of 0, 2.5, 5, 10, 25 ppm mustard controls
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution
  7. 5 pouches of Veratox mustard allergen extraction buffer. Each pouch is enough to prepare 1 L in distilled or deionized water.
  8. 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  9. 50 g of extraction additive in a specimen cup
  10. Plastic scoop to measure extraction additive
  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable plastic extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile sawbs
    2. 100 dropper tips
  3. Shaker water bath capable of maintaining 60 ± 1°C with clamps to hold 250 mL extraction bottles
  4. Whatman no. 4 filters or equivalent (Neogen item 9429)
  5. Centrifuge (optional)
  6. Filter funnel, 65 mm (Neogen item 9353)
  7. Pipettor, 50–200 µL, adjustable (Neogen item 9276)
  8. Pipettor, 12-channel (Neogen item 9273)
  9. Pipette tips (Neogen item 9410, 9407, 9417)
  10. Timer (Neogen item 9426)
  11. Microwell reader with a 650 nm filter (Neogen item 9303)
  12. Bottle, 1 L, to prepare washing solution (Neogen item 9472)
  13. Bottle, 1 L heat safe, to prepare extract solution (Neogen item 9472)
  14. Paper towels or equivalent absorbent material
  15. Microwell holder (Neogen item 9402)
  16. Waterproof marker
  17. Wash bottle (Neogen item 9400)
  18. Distilled or deionized water
  19. 3 reagent boats for 12-channel pipettor (Neogen item 9435)
  20. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  21. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)

Información adicional

A Guide to Risk Assessment Using Food Allergen Reference Doses

Tecnología

The Veratox for Mustard Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Mustard protein residue is extracted from samples with a Tris-EDTA solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted mustard protein is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound mustard protein is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound mustard protein. After a second wash, substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of mustard, expressed as ppm mustard.

Capacitación

El éxito de nuestros clientes es un éxito compartido. Nuestros expertos están listos para capacitarlo a usted y a su equipo sobre nuestras soluciones, de forma que pueda estar seguro de que los procedimientos se realizan correctamente y generan resultados exactos. Además, le proporcionamos certificados al finalizar la capacitación para brindarle la documentación que necesita con fines de auditoría.

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