Colorimetric and Chemiluminescent HRP Substrates for Reliable ELISA Results
Neogen® manufactures an expanding range of colorimetric and chemiluminescent substrates designed for horseradish peroxidase (HRP)‑based microwell applications. Every K‑Blue® formula offers excellent sensitivity, stability, and lot‑to‑lot consistency, with a linear kinetic response across a range of analyte concentrations.
Designed for immunoassay manufacturing and research laboratories, K‑Blue delivers the reliable performance needed for ELISA development and production‑scale assay workflows — supported by a partner you can count on.
Neogen is a trusted partner known for our:
- Proven track record with over 20 years of manufacturing excellence and consistent quality.
- Reliable supply chain supported by U.S.-based manufacturing and global distribution.
- Production support strengthened by custom packaging solutions to streamline your workflow.
K‑Blue: Built to Support Your Assay From Development to Scale‑Up
When you're developing or optimizing an ELISA, the details matter. K‑Blue substrates are engineered to remove the friction points that slow development, introduce variability, or complicate regulatory readiness. Our team is here to help you overcome common challenges such as:
- Lot‑to‑lot consistency ensured through our controlled manufacturing process and rigorous QC testing of every batch.
- Closing regulatory gaps through a robust, ISO‑certified Quality Management System that supports customer success in audits and approvals.
- Managing cost and inventory pressures with long shelf‑life formulas that protect your intended kit dating.
- Small‑to‑large scale capability with various off‑the‑shelf sizes and large batches available.
K‑Blue substrates are purpose‑built to protect your assay performance, support your regulatory needs, and provide a stable, scalable solution as your product grows.
The K-Blue Advantages
K‑Blue is built on four core advantages—performance, stability, quality, and compliance—delivering consistent, trusted performance in every ELISA.
Performance
- Multiple formulas offer a range of kinetic rates and sensitivity levels, allowing selection of the most appropriate formula for your application.
Stability
- Shelf‑life ranging from 24 to 48 months.
- Stability is monitored in real‑time with stringent quality control standards.
- Minimal activity loss over the course of the shelf‑life.
- Consistent performance across typical shipping and storage conditions.
Quality
- ISO 9001:2015 certified, our quality management system improves our efficiency and effectiveness, ensuring our customers receive consistent high‑quality products.
Compliance
- Formulas are Globally Harmonized System (GHS) compliant.
- 100% solvent‑free formula available.
- Formulas that meet strict regulatory environments.
- REACH compliant options available.
The Story Behind K‑Blue
K‑Blue takes its name from the deep Kentucky Blue color — Pantone® 286 — that forms in the presence of peroxidase, a nod to its roots in Lexington, Kentucky, USA, where it's still manufactured today.
Since its introduction, K‑Blue has become a trusted substrate across thousands of Neogen‑manufactured assays, including Veratox® natural toxin and allergens ELISA tests, and a full range of toxicology products. Beyond Neogen, end users rely on the K‑Blue portfolio of substrates for a wide range of ELISA and immunoassay applications including clinical, veterinary, food safety, environmental, and others.
Substrates & Reagents Brochure
Discover Neogen’s high‑performance TMB substrates engineered for sensitive, consistent detection across HRP-based immunoassays.
Custom Packaging
We can package substrates and reagents in custom bottle sizes and volume fills to meet your specific packaging requirements. Our fully automated filling system helps ensure consistent fill accuracy, product quality, and manufacturing efficiency.
Custom‑filling capabilities:
- Fill volumes 6 mL to 20 L
- Bottle sizes from 15 mL bottles to 20 L containers
- Tailored labeling options
Custom-filling benefits:
- Reduces risk of substrate contamination
- Minimizes exposure to light and air
- Ensures long‑term stability of the substrate
- Precise volume fill per bottle reduces volume variability bottle‑to‑bottle
- Reduced cost from overfilling
Discover the K-Blue Portfolio
K-Blue is a portfolio of colorimetric and chemiluminescent substrates designed for HRP-based microwell applications. For a full overview of Neogen’s complete immunoassay reagent portfolio, visit our Immunoassay Reagents solutions page or our Immunoassay Manufacturing page.
Colorimetric Substrate Products
Chemiluminescent Substrate Products
Additional Resources
K-Blue Luminescent ONE
This is our newest addition to the K‑Blue Chemiluminescent product line. K‑Blue Luminescent ONE is specifically designed as a one‑bottle, ready‑to‑use solution to simplify your workflow, saving time and improving efficiency.
K-Blue Advanced Plus
K‑Blue Advanced Plus is a one‑bottle, ready‑to‑use solution that delivers high activity, low background, long‑term stability, and full compliance with the REACH Restricted Substances List Annex XVII. With no special labeling requirements and consistently strong performance, it is an ideal choice for manufacturers’ immunoassay production needs.