Megazyme® Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)

800 Units

SKU No.  700004223  |  Catalog No.  E-ISAMYFO-800U

AOAC OMA

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
Approvals

AOAC OMA 2000.11

EC Number

3.2.1.68

Storage Conditions

Storage Temperature: 4°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food and feeds, and cereals industry.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

9067-73-6

Concentration~ 200
Synonyms

isoamylase; glycogen 6-alpha-D-glucanohydrolase

SourceFlavobacterium odoratum
Molecular Weight

83000

ExpressionPurified from Flavobacterium odoratum
Specificity

Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Specific Activity

~ 140 U/mg (40°C, pH 6.0 with 1 mM CaCl2 on oyster glycogen)

Unit Definition

One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40°C.

Temperature Optima (°C)50
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.03 lb

Documents

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