Isoamylase (Glycogen 6-glucanohydrolase)

600 Units

SKU No.  700004222  |  Catalog No.  E-ISAMY

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Please refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.

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Specifications
BrandMegazyme
ApprovalsAOAC Method 2000.11 and GB Standard 5009.245-2016
EC Number3.2.1.68
EnzymeIsoamylase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food and feeds, and cereals industry.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9067-73-6
CAZy FamilyGH13
Concentration~ 200
Synonymsisoamylase; glycogen 6-alpha-D-glucanohydrolase
SourcePseudomonas sp.
Molecular Weight71500
ExpressionPurified from Pseudomonas sp.
SpecificityHydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity~ 180 U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).
Unit DefinitionOne unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°C.
Temperature Optima (°C)50
pH Optima4
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.03 lb