Megazyme® Isoamylase (Glycogen 6-glucanohydrolase)

600 Units

SKU No.  700004222  |  Catalog No.  E-ISAMY

AOAC OMA

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and analytical testing applications.

Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are utilised in the enzyme hydrolysis step of methods for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Please refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.

View Documents

Specifications
BrandMegazyme®
Approvals

AOAC OMA 2000.11

EC Number

3.2.1.68

Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food and feeds, and cereals industry.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

9067-73-6

Concentration~ 200
Synonyms

isoamylase; glycogen 6-alpha-D-glucanohydrolase

SourcePseudomonas sp.
Molecular Weight

71500

ExpressionPurified from Pseudomonas sp.
Specificity

Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Specific Activity

~ 120 U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg)

Unit Definition

One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°C.

Temperature Optima (°C)50
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.03 lb