Megazyme® Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

SKU No.  700004221  |  Número de catálogo  E-HKGDH

Cantidad:
Cantidad mínima necesaria: 1
É necessário fazer o pedido do item em múltiplos de uma caixa. Quantidade por caixa: 1

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and analytical testing applications.

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Especificaciones
MarcaMegazyme
Número CE

Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49

Condiciones de almacenamiento

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Ejemplos de aplicaciónApplications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
FormulaciónIn 3.2 M ammonium sulphate
Forma físicaSuspension
Número CAS

Hexokinase: 9001-51-8
G6P-DH: 9001-40-5

ConcentraciónHexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Sinónimos

Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

FuenteYeast
ExpresiónHexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
especificidad

Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Definición de unidad

Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

Temperatura Óptima (°C)40
Dimensiones del empaque0,80 pulgada x 0,80 pulgada x 1,90 pulgada
Peso del paquete0,06 libra