MiSeq Reagent Kit v3 (150-cycle)
SKU Nr.  11504  | Katalognummer 
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MiSeq Reagent Kit v3 (150-cycle)

SKU Nr.  11504  |  Katalognummer 

  • Max read length of 2 x 75 bp.
  • Common for transcriptome analysis.
  • 20–25 million read-pairs.
  • Estimated output of 3.3–3.8 Gb.
  • FASTQC report included with all prepared pool orders.
  • All prepared pools will be QC'd at Neogen. If the results indicate poor quality, we will contact you.
  • 4 week turnaround time.
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To order or for more information contact us.
To order or for more information contact us.
The Illumina MiSeq benchtop sequencer is ideal for de novo assembly of small genomes or amplicon sequencing projects. Illumina MiSeq Reagent Kits v3 contain improved chemistry to increase cluster density and read depth as well as improve quality (Q) scores. The 150-cycle v3 flow cell enables a max read length of 2 x 75 bp, with approximately 20–25 million read-pairs. Each prepared pool order will include a FASTQC report for the sequencing run.
Spezifikationen
MarkeIllumina
PlattformSequenzierung
Informationen zur Übermittlung50 uL of your prepared pool at a concentration of 5 nM, determined by fluorometry or qPCR, is required. (the u in uL should be the micro symbol). Please ship your 5 nM pool in a screw cap tube within a para-filmed 50 mL centrifuge tube at the requested volume on dry ice to Neogen Genomics, 4131 N 48th St., Lincoln, NE 68504.
Bearbeitungszeit4 Weeks
FASTQ and FASTQC delivery via AWS

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Q: What should the concentration of the prepared pools be for submission?

A: Prepared pools should be submitted to Neogen at a concentration of 5 nM, determined by fluorometry or qPCR.

Q: How much volume of the prepared pool is needed for sequencing?

A: MiSeq runs require 50 uL of the prepared pool. NovaSeq runs require 4 unique pools of 100 uL.

Q: Where and how should the prepared pools be shipped?

A: Please ship your 5 nM prepared pool in a screw cap tube within a para-filmed 50 mL centrifuge tube at the requested volume on dry ice to Neogen Genomics, 4131 N 48th St., Lincoln, NE 68504.

Q: How will sequencing and QC data be delivered?

A: Data will be delivered via Amazon Web Services (AWS).

Q: Will Neogen perform library pooling?

A: No. At this time, Neogen will not perform library pooling. Libraries must be pooled before submitting for sequencing.