Megazyme® Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

SKU Nr.  700004221  |  Katalognummer  E-HKGDH

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High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and analytical testing applications.

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Spezifikationen
MarkeMegazyme
EC-Nummer

Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49

Lagerbedingungen

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

AnwendungsbeispieleApplications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
FormulierungIn 3.2 M ammonium sulphate
Physische FormSuspension
CAS-Nummer

Hexokinase: 9001-51-8
G6P-DH: 9001-40-5

KonzentrationHexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Synonyme

Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

QuelleYeast
AusdruckHexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Spezifität

Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Einheitendefinition

Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

Temperaturoptima (°C)40
Abmessungen der Packung0,80 Zoll x 0,80 Zoll x 1,90 Zoll
Packungsgewicht0,06 lb