Megazyme® Fructanase Mixture (Ultrapure, recombinant, powder)
SKU No.  700004217  | Catalog No.  E-FRPDPU
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Megazyme® Fructanase Mixture (Ultrapure, recombinant, powder)

20,000 Units

SKU No.  700004217  |  Catalog No.  E-FRPDPU

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Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact our food safety team
To order or for more information contact our food safety team

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Components: exo-Inulinase 20,000 U (on kestose at 40°C), endo-inulinase 1000 U (on fructan at 40°C).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

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Specifications
BrandMegazyme®
EC Number

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Storage Conditions

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions

FormulationSupplied as a freeze-dried powder
Physical FormPowder
CAS Number

9001-57-4,
9025-67-6,
37288-56-5

Concentrationendo-inulinase: Supplied at ~ 100 U/mL
exo-inulinase: Supplied at ~ 2,000 U/mL
Synonyms

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

SourceAspergillus niger
ExpressionRecombinant from Aspergillus niger
Specificity

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Unit Definition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Temperature Optima (°C)60
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.06 lb