Megazyme® Fructanase Mixture (Ultrapure, recombinant, powder)
20,000 Units
SKU 编号 700004217 | 目录编号 E-FRPDPU
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Components: exo-Inulinase 20,000 U (on kestose at 40°C), endo-inulinase 1000 U (on fructan at 40°C).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
| 规格 | ||
|---|---|---|
| 品牌 | Megazyme | |
| EC 编号 | endo-Inulinase: 3.2.1.7, | |
| 储存条件 | Storage Temperature: Below -10°C Stability: > 1 year under recommended storage conditions | |
| 配方 | Supplied as a freeze-dried powder | |
| 外形 | Powder | |
| CAS 编号 | 9001-57-4, | |
| 浓度 | endo-inulinase: Supplied at ~ 100 U/mL exo-inulinase: Supplied at ~ 2,000 U/mL | |
| 同义词 | endo-inulinase: 1-beta-D-fructan fructanohydrolase | |
| 来源 | Aspergillus niger | |
| 表达 | Recombinant from Aspergillus niger | |
| 特异性 | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. | |
| 单位定义 | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. | |
| 最佳温度 (°C) | 60 | |
| 包装尺寸 | 0.80 in x 0.80 in x 1.90 in | |
| 包装重量 | 0.06 lb | |