Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)
SKU No.  700004214  | Catalog No.  E-FRLQPU
Top

Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)

10 ml / 20,000 Units

SKU No.  700004214  |  Catalog No.  E-FRLQPU

Learn more
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact our food safety team
To order or for more information contact our food safety team

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Components: exo-Inulinase (2,000 U/mL on kestose, at 40°C) and endo-Inulinase (100 U/mL on inulin at 40°C).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

View Documents

Megazyme_Scheme-E-FRLQPU.png
Specifications
BrandMegazyme®
EC Number

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Storage Conditions

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

Application ExamplesApplications in carbohydrate research and in the food industry.
FormulationIn 50% (v/v) glycerol plus 0.02% sodium azide
Physical FormSolution
CAS Number

9001-57-4,
9025-67-6,
37288-56-5

Concentrationendo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 10,000 U
Synonyms

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

SourceAspergillus niger
ExpressionRecombinant from Aspergillus niger
Specificity

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Unit Definition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Temperature Optima (°C)60
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.06 lb