Megazyme® Fructanase Mixture (powder)

20,000 Units

SKU Nr.  700004216  |  Katalognummer  E-FRMXPD

AOAC OMA

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Erforderliche Mindestmenge: 1
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High purity Fructanase Mixture (powder) for use in research, biochemical enzyme assays and analytical testing applications.

Components: exo-Inulinase 20,000 U (on kestose at 40°C), endo-inulinase 1000 U (on fructan at 40°C).

Fructanase, along with Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY), can also be utilised in the enzyme hydrolysis step for determining polydextrose a low molecular weight dietary fiber in food samples.

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan and Polydextrose.

Megazyme_Scheme-E-FRMXPD.png
Spezifikationen
MarkeMegazyme
Akkreditierungen

For use with AOAC OMA 997.08, 2000.11, 2016.06

EC-Nummer

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Lagerbedingungen

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

FormulierungSupplied as a lyophilised powder
Physische FormPowder
CAS-Nummer

9001-57-4,
9025-67-6,
37288-56-5

Synonyme

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

QuelleAspergillus sp.
AusdruckFrom Aspergillus sp.
Spezifität

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Einheitendefinition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Temperaturoptima (°C)40
Abmessungen der Packung0,80 Zoll x 0,80 Zoll x 1,90 Zoll
Packungsgewicht0,03 lb