Megazyme® Fructanase Mixture (liquid)
SKU Nr.  700004215  | Katalognummer  E-FRMXLQ
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Megazyme® Fructanase Mixture (liquid)

10 ml / 20,000 Units

SKU Nr.  700004215  |  Katalognummer  E-FRMXLQ

AOAC OMA

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Erforderliche Mindestmenge: 1
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To order or for more information contact our food safety team
To order or for more information contact our food safety team

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.

Components: exo-inulinase 2,000 U/ml (on kestose, at 40°C), endo-inulinase ˜ 100 U/ml (on fructan at 40°C), α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40°C), β-Glucanase < 0.04 U/ml (on β-glucan at 40°C) and pectinase < 0.07 U/ml (on pectin at 40°C).

Fructanase, along with Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY), can also be utilised in the enzyme hydrolysis step for determining polydextrose a low molecular weight dietary fiber in food samples.

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan and polydextrose.

Fructanase and sucrase (E-SUCR) may also be utilized in the enzymatic hydrolysis of fructans during analytical procedures designed to quantify fructan content in food samples according to the relevant AOAC and GB standard methods.

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Spezifikationen
Marke Megazyme
Akkreditierungen

For use with AOAC OMA 997.08, 2000.11, 2016.06
EC-Nummer

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
Lagerbedingungen

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details
Formulierung In 50% (v/v) glycerol
Physische Form Solution
CAS-Nummer

9001-57-4,
9025-67-6,
37288-56-5
Synonyme

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Quelle Aspergillus sp.
Ausdruck From Aspergillus sp.
Spezifität

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Einheitendefinition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.
Temperaturoptima (°C) 40
Abmessungen der Packung 0,80 Zoll x 0,80 Zoll x 1,90 Zoll
Packungsgewicht 0,06 lb