Enzyme Activity Analysis

Subcategory

Dyed Substrates
Enzyme Activity Assay Kits
Oligosaccharides & Colourimetric Substrates
Polysaccharides
Reagents
Sugars
Tablet Tests

Analyte

1,4-β-D-Glucosaminidase
Amyloglucosidase
Catalase
Cellobiohydrolase
Chitin Deacetylase
Chitobiase
Chitosanase
Esterase
Glucose Oxidase
Hexosaminidase

Result Type

Fluorescence
UV Absorbance

Platform

Auto-analyser
Microplate
Petri-Dish
Spectrophotometer

Brand

Megazyme

Validation Method

AACC Method 22-02.01
AOAC Method 2002.01
ASBC Method Malt 7
ASBC Method Wort 18
EBC Method 3.10.2
EBC Method 4.13
EBC Method 4.16.2
EBC Method 8.13.2
EBC Method 8.13.3
EBC Method 9.31.2
Filters
Results 257
Xylazyme Tablets
High purity dyed and crosslinked Xylazyme (100 mg tablets) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase
Platform: Spectrophotometer
Result Type: UV Absorbance
ItemItem No.Unit Price
200 TabletsT-XYZ-200T

$484.96

α-Amylase Reagent (Ceralpha)
Item No. R-CAAR4
Purchase high purity Ceralpha: α-Amylase Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis. 
Analyte: α-Amylase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
$510.72 each
α-Amylase Assay Kit (Ceralpha Method)
Item No. K-CERA
α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
Analyte: α-Amylase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
Approvals: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018)
$364.00 each
α-Amylase HR Reagent
Item No. R-AMHR4
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: α-Amylase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
$539.84 each
β-Glucan (Barley; Medium Viscosity)
Item No. P-BGBM
High purity β-Glucan (Barley; Medium Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, β-Glucanase, Lichenase
Approvals: EBC Method 8.13.3 and ASBC Method Wort 18
$428.96 each
Cellulase Assay Kit (CellG5 Method)
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase).
Analyte: endo-Cellulase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 1.2 x 10-3 U/mL
Approvals: Novel method
ItemItem No.Unit Price
120 / 240 assays (manual) / 480 assays (auto-analyser)K-CELLG5-4V

$500.64

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Xylanase Assay Kit (XylX6 Method)
The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase).
Analyte: endo-1,4-α-Xylanase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 5.3 x 10-4 U/mL
Approvals: Novel method
ItemItem No.Unit Price
200 assays (manual) / 400 assays (auto-analyser)K-XYLX6-2V

$500.64

Arabinoxylan (Wheat Flour; Medium Viscosity)
Item No. P-WAXYM
High purity Arabinoxylan (Wheat Flour; Medium Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, α-Arabinofuranosidase
$249.76 each
Xylazyme AX Tablets
High purity dyed and crosslinked Xylazyme AX (60 mg tablets) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase
Platform: Spectrophotometer
Result Type: UV Absorbance
Approvals: RACI Standard Method
ItemItem No.Unit Price
200 TabletsT-XAX-200T

$420.00

Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)
Item No. K-PULLG6
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and
Analyte: Pullulanase, Limit-dextrinase
Platform: Spectrophotometer
Result Type: UV Absorbance
Limit of Detection: 0.18 U/mL for pullulanase preparations (50-fold dilution), 0.01 U/g for limit dextrinase in milled malt
Approvals: Novel method
$424.48 each
β-Limit Dextrin 
High purity β-Limit Dextrin 10 g for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: α-Amylase, Pullulanase, Limit-dextrinase
Approvals: EBC Method 4.13 and ASBC Method Malt 7
ItemItem No.Unit Price
10 gP-BLDX-10G

$201.60

β-Amylase Assay Kit (Betamyl-3)
Item No. K-BETA3
The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.
Analyte: α-Amylase
Platform: Spectrophotometer
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
Approvals: Modification of RACI Standard Method
$375.20 each
β-Amylase Assay Reagent (Betamyl-3)
Item No. R-BAMR3
High purity Betamyl-3; β-Amylase Assay Reagent – 4 vials for the measurement of β-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: β-Amylase
Platform: Spectrophotometer
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
$534.24 each
Maltotriose
Item No. O-MAL3
High purity Maltotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: Amyloglucosidase, α-Glucosidase, β-Amylase
$272.16 each
Xylan (Beechwood)
Highly purified xylan from beechwood for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase
ItemItem No.Unit Price
10 g P-XYLNBE-10G

$201.60

β-Glucan CFA Standard 
Item No. P-BGCFA
High purity β-Glucan CFA Standard (previously FIA Standard) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, β-Glucanase, Lichenase
Approvals: EBC Method 3.10.2, EBC Method 4.16.2, EBC Method 8.13.2, EBC Method 9.31.2 and ASBC Method Wort 18
$240.80 each
Protazyme AK Tablets
endo-Protease enzyme activity assay using Protozyme AK tablets. Containing AZCL-Casein as a substrate. High sensitivity.
Analyte: Protease
Platform: Spectrophotometer
Result Type: UV Absorbance
ItemItem No.Unit Price
200 TabletsT-PRAK-200T

$420.00

Xylohexaose
Item No. O-XHE
High purity Xylohexaose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 
Analyte: endo-1,4-β-Xylanase, β-Xylosidase
$268.80 each
α-Amylase SD Assay Kit
Item No. K-AMYLSD
The Amylase SD Method is a highly sensitive colourimetric method for the determination of α-amylase in sprout damaged wheat grain (also known as pre-harvest sprouting or weather damaged wheat grain) and “late maturity α-amylase” wheat grain.
Analyte: α-Amylase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
$362.88 each
β-Glucan (Barley; Low Viscosity)
Item No. P-BGBL
High purity β-Glucan (Barley; Low Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, β-Glucanase, Lichenase
$411.04 each
Xylotriose
Item No. O-XTR
High purity Xylotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, β-Xylosidase
$247.52 each
Mannazyme Tablets
High purity dyed and crosslinked Mannazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Mannanase
Platform: Spectrophotometer
Result Type: UV Absorbance
ItemItem No.Unit Price
200 TabletsT-MNZ-200T

$420.00

Xylobiose
Item No. O-XBI
High purity Xylobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 
Analyte: β-Xylosidase
$247.52 each
Azocasein
Item No. S-AZCAS
High purity dyed, soluble Azocasein (Sulphanilamide Dyed) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: Protease
Platform: Spectrophotometer, Petri-Dish
Result Type: UV Absorbance
$249.76 each
Xylopentaose
Item No. O-XPE
High purity Xylopentaose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, β-Xylosidase
$202.72 each
Xylotetraose
Item No. O-XTE
High purity Xylotetraose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, β-Xylosidase
$247.52 each
Cellazyme C Tablets
High purity dyed and crosslinked Cellazyme C tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase
Platform: Spectrophotometer
Result Type: UV Absorbance
ItemItem No.Unit Price
200 TabletsT-CCZ-200T

$411.04

Cellazyme T Tablets
High purity dyed and crosslinked Cellazyme T tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, Xyloglucanase
Platform: Spectrophotometer
Result Type: UV Absorbance
ItemItem No.Unit Price
200 TabletsT-CTZ-200T

$424.48

Amyloglucosidase Assay Reagent 
Item No. R-AMGR3
High purity Amyloglucosidase Assay Reagent – 4 vials for the measurement of amyloglucosidase, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: Amyloglucosidase
Platform: Spectrophotometer
Result Type: UV Absorbance
Limit of Detection: ~ 2.5 U/mL
$469.28 each
Azo-Xylan (Birchwood) (Powder)
Item No. S-AXBP
High purity dyed, soluble Azo-Xylan (Birchwood) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase
Platform: Spectrophotometer, Petri-Dish
Result Type: UV Absorbance
$299.04 each
Xyloglucan (Tamarind)
Item No. P-XYGLN
High purity Xyloglucan (Tamarind) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, Xyloglucanase
$207.20 each
Red Pullulan
Item No. S-RPUL
High purity dyed, soluble Red Pullulan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: Pullulanase, Limit-dextrinase, Isopullulanase, Neopullulanase, Isoamylase
Platform: Spectrophotometer, Petri-Dish
Result Type: UV Absorbance
$299.04 each
Mannotriose
Item No. O-MTR
High purity Mannotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Mannanase, β-Mannosidase
$247.52 each
β-Glucazyme Tablets
High purity dyed and crosslinked β-Glucazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, β-Glucanase, Lichenase
Platform: Spectrophotometer
Result Type: UV Absorbance
ItemItem No.Unit Price
200 TabletsT-BGZ-200T

$378.56

Amylazyme Tablets
High purity dyed and crosslinked Amylazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: α-Amylase
Platform: Spectrophotometer
Result Type: UV Absorbance
Approvals: AACC Method 22-05.01, RACI Standard Method
ItemItem No.Unit Price
200 TabletsT-AMZ-200T

$311.36

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
Item No. K-MBG4
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4).
Analyte: Lichenase
Platform: Spectrophotometer, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL
Approvals: Novel method
$388.64 each
β-Glucan (Barley; High Viscosity)
Item No. P-BGBH
High purity β-Glucan (Barley; High Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, β-Glucanase, Lichenase
$454.72 each
Arabinoxylan (Wheat Flour; Low Viscosity)
Item No. P-WAXYL
High purity Arabinoxylan (Wheat Flour; Low Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, α-Arabinofuranosidase
$249.76 each
Arabinoxylan (Wheat Flour; High Viscosity)
Item No. P-WAXYH
High purity Arabinoxylan (Wheat Flour; High Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, α-Arabinofuranosidase
$290.08 each
Glucose Oxidase Assay Kit
Item No. K-GLOX
The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.
Analyte: Glucose Oxidase
Platform: Spectrophotometer, Microplate, Auto-analyser
Result Type: UV Absorbance
Limit of Detection: 10 U/L
Approvals: Novel method
$211.00 each
Azo-Wheat Arabinoxylan (Powder)
Item No. S-AWAXP
High purity dyed, soluble Azo-Wheat Arabinoxylan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase
Platform: Spectrophotometer, Petri-Dish
Result Type: UV Absorbance
$341.60 each
CM-Curdlan
Item No. P-CMCUR
High purity CM-Curdlan for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,3-β-Glucanase
$249.76 each
Cellohexaose
Item No. O-CHE
High purity Cellohexaose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, Cellobiohydrolase, β-Glucosidase
$238.56 each
Galactomannan (Carob; Low Viscosity)
Item No. P-GALML
High purity Galactomannan (Carob; Low Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Mannanase, α-Galactosidase
$247.52 each
Maltotetraose
Item No. O-MAL4
High purity Maltotetraose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: α-Amylase, β-Amylase, α-Glucosidase, Amyloglucosidase
$255.36 each
Mannobiose
Item No. O-MBI
High purity Mannobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: β-Mannosidase
$247.52 each
Arabinoxylan (Rye Flour)
Item No. P-RAXY
High purity Arabinoxylan (Rye Flour) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-1,4-β-Xylanase, α-Arabinofuranosidase
$249.76 each
Azo-Barley Glucan
Item No. S-ABG100
High purity dyed, soluble Azo-Barley Glucan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Analyte: endo-Cellulase, β-Glucanase, Lichenase
Platform: Spectrophotometer, Petri-Dish
Result Type: UV Absorbance
$268.80 each
Malt Amylase Assay Kit
Item No. K-MALTA
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
Analyte: α-Amylase, β-Amylase
Platform: Spectrophotometer
Result Type: UV Absorbance
Limit of Detection: 0.05 U/mL
Approvals: “Ceralpha” Method: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018). “Betamyl-3” Method: RACI Standard Method.
$367.36 each
Panose
High purity Panose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Panose is formed by the action of neopullulanase on pullulan.
Analyte: Amyloglucosidase, α-Glucosidase
ItemItem No.Unit Price
1 gO-PAN-1G

$509.60

Enzymes’ importance in a wide range of manufacturing processes and scientific research is constantly growing. Effective methods to measure enzyme activities are paramount for enzyme characterization and the discovery and development of new enzyme preparations.

Megazyme has unrivaled expertise in diagnostic solutions to measure enzymatic activity using sensitive and user-friendly assay methods that are automatable in some cases. Our wide range of diverse enzyme activity products is available in several formats: assay kits, tablets, colorimetric substrates, ultrapure oligosaccharides, and polysaccharides. These products can analyze diverse sample types, from purified enzymes to crude enzyme preparations or complex food matrices.