Veratox® VIP for Peanut

Item No.  8430M

  • Features an optimized antibody combination and extraction procedure for high specificity to all types of peanut protein, particularly further-processed peanut.
  • Highly sensitive, quantifying peanut protein down to 0.25 ppm, aligned with most up-to-date industry guidelines.
  • Rapid and easy to use, with a 30 minute time to result, a simple testing procedure consistent across sample types, and ready to use, non-hazardous reagents.
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$749.33 each
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Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Veratox® VIP (Veratox Improvement Platform) for Peanut Allergen offers enhanced quantitative analysis of very low levels of raw and further-processed peanut residues in a wide variety of food products, ingredients, and clean-in-place rinses. This product can also be used to screen environmental swabs.
Specifications
Brand Veratox®
Analyte Peanut
Platform ELISA
Result Type Quantitative
Limit of Detection .08 ppm
Range of Quantitation 0.25 ppm peanut - 5.00 ppm peanut
Testing Time 30.00 Minutes
Storage Conditions Store refrigerated
Quantity per Package 48 wells
  1. 48 antibody-coated microwells
  2. 48 red-marked transfer wells
  3. 6 yellow-labeled bottles of 0, 0.25, 0.625, 1.25, 2.5, and 5 ppm peanut protein controls (0, 1, 2.5, 5, 10, and 20 ppm total peanut)
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop Solution
  7. 5 foil pouches of 10 mM PBS dry powder extraction diluent. Each pouch is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  8. 40 mL of 10 mM PBS-tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  9. Two 100 μL tubes of Reagent A conjugate enhancement solution
  10. 200 g extraction additive
    Note: Only for use with samples containing high levels of polyphenols/tannins (e.g., chocolate)
  1. Allergen extraction kit (Neogen® item 8429)
    1. 20 disposable plastic extraction bottles
    2. 20 sample collection tubes (12 x 75 mm) with caps
  2. Shaker water bath capable of maintaining 60°C ± 1° with clamps to hold 250 mL extraction bottles (Neogen items 9298, 9299)
  3. Whatman #4 filters or equivalent (Neogen item 9429)
  4. Centrifuge (optional)
  5. Pipettor, 50–200 μL adjustable (Neogen item 9276)
  6. Pipettor, 12-channel pipettor (Neogen item 9273)
  7. Pipette tips (Neogen items 9410 , 9407, 9417)
  8. Timer (Neogen item 9426)
  9. Stat-Fax 4700 microwell reader with a 650 nm filter (Neogen item 9303)
  10. 1 L bottle to prepare washing solution (Neogen item 9472)
  11. 1 L bottle to prepare extraction buffer diluent (Neogen item 9472)
  12. Paper towels or equivalent absorbent material
  13. Microwell holder (Neogen item 9402)
  14. Waterproof marker
  15. Wash bottle (Neogen item 9400)
  16. Distilled or deionized water
  17. 3 reagent boats for 12-channel pipettor (Neogen item 9435)
  18. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  19. Scale capable of weighing 5 g ± 0.1 g (Neogen item 9427)

Documents

To access the Certificate of Analysis (COA), please use our COA Search.

Technology

The Veratox VIP for Peanut test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). This assay is validated to detect three primary allergenic proteins in peanuts (Ara h 1, Ara h 2, and Ara h 3). Peanut protein is extracted from samples with a phosphate-buffered salt (PBS) based solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted peanut protein is sampled and added to antibody-coated wells (capture antibody), where it binds to the antibody during an incubation. Any unbound peanut protein is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound peanut protein. After a second wash, substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop Solution reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities (OD). The OD of the controls form a standard curve, and the sample OD are plotted against the curve to calculate the concentration of peanut protein.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

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