Veratox® for Gliadin R5

SKU No.  700002592  |  Catalog No.  8510

  • Accurate and easy-to-use
  • Quantitate or screen samples
  • High specificity
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$772.00 each
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Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Veratox® for Gliadin R5 is used for the quantitative analysis of ingredients, clean-in-place rinses and finished food products intended to be gluten free for the presence of gliadin and prolamins found in wheat, barley and rye. Can also be used to screen environmental swabs.
Specifications
Brand Veratox®
Analyte Gliadin/Gluten
Platform ELISA
Result Type Quantitative
Approvals AOAC-RI 061201
Limit of Detection 2.5 ppm gliadin
Testing Time 30.00
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 48 tests
Package Dimensions 10.50 in x 8.00 in x 3.00 in
Package Weight 0.90 lb
  1. 48 antibody-coated microwells
  2. 48 red-marked transfer wells
  3. 6 yellow-labeled bottles of 0, 2.5, 5, 10, 20, and 40 ppm gliadin controls (0, 5, 10, 20, 40, and 80 ng/mL gliadin)
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution
  7. 40 mL of 10 mM PBS-Tween washing reagent in a widemouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
  8. 1 foil pouch sample diluent concentrate of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution buffer
  9. 50 g of extraction additive in 2 specimen cups
  10. Plastic scoop to measure extraction additive
  1. Microwell Reader with 650 nm filter (Neogen® item 9303)
  2. Graduated cylinder 10–20 mL (Neogen item 9447)
  3. Graduated cylinder 250 mL (Neogen item 9368)
  4. 1–Liter bottle (Neogen item 9472)
  5. Pipettor, 50-200 µL adjustable (Neogen item 9276)
  6. Pipettor, 12-channel 50-200 µL ((Neogen item 9273)
  7. Pipette tips (Neogen item 9410, 9407, 9417)
  8. Wash bottle (Neogen item 9400)
  9. Well holder (Neogen item 9402)
  10. Timer (Neogen item 9426)
  11. Reagent boats, set of 3 (Neogen item 9435)
  12. 7 mL tube or vial (Neogen item 9468)
  13. Pipette pump for serological tubes (Neogen item 9277)
  14. Pipettes, sterile serological, 10 mL (pk of 100) (Neogen item 9415)
  15. Laboratory grade ethanol (190 proof)
  16. Distilled water
  17. Waterproof marker
  18. Paper towel or equivalent
  19. Centrifuge (optional)
  20. Vortex (optional)

Non-heat treated method (extraction A or B)

  1. Tube, 50 mL (Neogen item 9381) or bottle, 125 mL (Neogen item 9398)
  2. Scale ± 0.1 g (Neogen item 9427, 9395)
  3. Orbital rotator (Neogen item 9396) or shaker to hold 50 mL tube

Heat-treated cocktail method (extraction C)

  1. Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8515, 8515B)
  2. Orbital rotator (Neogen item 9396)
  3. Scale, ± 0.01g (Neogen Item 9395)
  4. Water bath or oven at 50°C

Documents

To access certificates, please use our technical document search page.

Veratox® for Gliadin R5 - English - US Veratox® for Gliadin R5 - Bulgarian - Bulgaria Veratox® for Gliadin R5 - Chinese Simplified - China Veratox® for Gliadin R5 - Chinese Traditional - Taiwan Veratox® for Gliadin R5 - Croatian - Croatia Veratox® for Gliadin R5 - Czech - Czech Republic Veratox® for Gliadin R5 - Danish - Denmark Veratox® for Gliadin R5 - Dutch - Belgium Veratox® for Gliadin R5 - Dutch - Netherlands Veratox® for Gliadin R5 - English - Australia Veratox® for Gliadin R5 - English - Canada Veratox® for Gliadin R5 - English - EU/UK Veratox® for Gliadin R5 - English - Great Britain Veratox® for Gliadin R5 - English - Ireland Veratox® for Gliadin R5 - English - Malta Veratox® for Gliadin R5 - English - New Zealand Veratox® for Gliadin R5 - English - Philippines Veratox® for Gliadin R5 - Estonian - Estonia Veratox® for Gliadin R5 - Finnish - Finland Veratox® for Gliadin R5 - French Veratox® for Gliadin R5 - French - Belgium Veratox® for Gliadin R5 - French - Canada Veratox® for Gliadin R5 - German Veratox® for Gliadin R5 - German - Austria Veratox® for Gliadin R5 - German - Luxembourg Veratox® for Gliadin R5 - Greek - Cyprus Veratox® for Gliadin R5 - Greek - Greece Veratox® for Gliadin R5 - Hungarian - Hungary Veratox® for Gliadin R5 - Indonesian - Indonesia Veratox® for Gliadin R5 - Italian - Italy Veratox® for Gliadin R5 - Japanese - Japan Veratox® for Gliadin R5 - Korean - Korea Veratox® for Gliadin R5 - Latvian - Latvia Veratox® for Gliadin R5 - Lithuanian - Lithuania Veratox® for Gliadin R5 - Malay - Malaysia Veratox® for Gliadin R5 - Maltese - Malta Veratox® for Gliadin R5 - Norwegian - Norway Veratox® for Gliadin R5 - Polish - Poland Veratox® for Gliadin R5 - Portuguese - Brazil Veratox® for Gliadin R5 - Portuguese - Portugal Veratox® for Gliadin R5 - Slovak - Slovakia Veratox® for Gliadin R5 - Slovene - Slovenia Veratox® for Gliadin R5 - Spanish - Colombia Veratox® for Gliadin R5 - Spanish - Mexico Veratox® for Gliadin R5 - Spanish - Mexico/Central America Veratox® for Gliadin R5 - Spanish - South America Veratox® for Gliadin R5 - Spanish - Spain Veratox® for Gliadin R5 - Spanish - US Veratox® for Gliadin R5 - Swedish - Sweden Veratox® for Gliadin R5 - Thai - Thailand Veratox® for Gliadin R5 - Vietnamese - Vietnam

Technology

Veratox for Gliadin R5 is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Gliadin is extracted from samples with a 60% ethanol solution by shaking in a shaker or rotator. The extract is diluted in Phosphate Buffered Saline (PBS) and diluted samples are added to R5 antibody-coated wells (capture antibody) where gliadin will bind to the antibody during an incubation period. Any unbound gliadin is washed away and a second antibody, which is R5 enzyme-labeled (detector antibody) is added. The detector antibody binds to the gliadin during another incubation period. Unbound enzyme-labeled antibody is washed away and a one stop substrate is added. Color develops as a result of the presence of bound-labeled antibody. A stopping reagent is added and the color of the solution is observed. Blue indicates samples containing high levels of gliadin while purple or red samples contain little or no gliadin. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of gliadin in parts per million (ppm).

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Support

Validation

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