MiSeq Reagent Kit v3 (150-cycle)
SKU No.  11504  | Número de catálogo 
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MiSeq Reagent Kit v3 (150-cycle)

SKU No.  11504  |  Número de catálogo 

  • Max read length of 2 x 75 bp.
  • Common for transcriptome analysis.
  • 20–25 million read-pairs.
  • Estimated output of 3.3–3.8 Gb.
  • FASTQC report included with all prepared pool orders.
  • All prepared pools will be QC'd at Neogen. If the results indicate poor quality, we will contact you.
  • 4 week turnaround time.
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$1,873.00 cada uno
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Cantidad mínima necesaria: 1
É necessário fazer o pedido do item em múltiplos de uma caixa. Quantidade por caixa: 1
To order or for more information contact us.
To order or for more information contact us.

The Illumina MiSeq benchtop sequencer is ideal for de novo assembly of small genomes or amplicon sequencing projects. Illumina MiSeq Reagent Kits v3 contain improved chemistry to increase cluster density and read depth as well as improve quality (Q) scores. The 150-cycle v3 flow cell enables a max read length of 2 x 75 bp, with approximately 20–25 million read-pairs. Each prepared pool order will include a FASTQC report for the sequencing run.

Pruebas disponibles

Las pruebas adicionales están disponibles para ser agregadas en el siguiente paso, al cargar el formulario de envío.

MiSeq Reagent Kit v3 (600-cycle)

The Illumina MiSeq v3 600-cycle kit generates 20–25 million read-pairs with an approximate output of 13.2–15 Gb.

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NovaSeq 6000 S4 Prepared Pool v1.5 (200 cycles)

The Illumina NovaSeq 6000 200-cycles prepared pool generates 16–20 billion paired-end reads (8–10 billion single-end reads), with an approximate output of 1600–2000 Gb.

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NovaSeq 6000 S4 Prepared Pool v1.5 (300 cycles)

The Illumina NovaSeq 6000 300-cycles prepared pool generates 16–20 billion paired-end reads (8–10 billion single-end reads), with an approximate output of 2400–3000 Gb.

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Especificaciones
MarcaIllumina
PlataformaSecuenciación
Información de envío50 uL of your prepared pool at a concentration of 5 nM, determined by fluorometry or qPCR, is required. (the u in uL should be the micro symbol). Please ship your 5 nM pool in a screw cap tube within a para-filmed 50 mL centrifuge tube at the requested volume on dry ice to Neogen Genomics, 4131 N 48th St., Lincoln, NE 68504.
Tiempo de respuesta4 Weeks
FASTQ and FASTQC delivery via AWS

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Q: What should the concentration of the prepared pools be for submission?

A: Prepared pools should be submitted to Neogen at a concentration of 5 nM, determined by fluorometry or qPCR.

Q: How much volume of the prepared pool is needed for sequencing?

A: MiSeq runs require 50 uL of the prepared pool. NovaSeq runs require 4 unique pools of 100 uL.

Q: Where and how should the prepared pools be shipped?

A: Please ship your 5 nM prepared pool in a screw cap tube within a para-filmed 50 mL centrifuge tube at the requested volume on dry ice to Neogen Genomics, 4131 N 48th St., Lincoln, NE 68504.

Q: How will sequencing and QC data be delivered?

A: Data will be delivered via Amazon Web Services (AWS).

Q: Will Neogen perform library pooling?

A: No. At this time, Neogen will not perform library pooling. Libraries must be pooled before submitting for sequencing.