Megazyme® Lactose Assay Kit - Sequential/High Sensitivity
65 assays per kit
SKU 编号 700004314 | 目录编号 K-LOLAC
AOAC OMA
- World’s first sequential assay for lactose, i.e. improves accuracy and efficiency
- Contains a specific β-galactosidase for the selective measurement of lactose in dairy products
- Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products
- Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1.62 mg/L
- Very competitive price (cost per test)
The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
| 规格 | ||
|---|---|---|
| 品牌 | Megazyme | |
| 分析物 | 乳糖 | |
| 平台 | 分光光度计 | |
| 结果类型 | 紫外吸光度 | |
| 批准 | AOAC OMA 2020.08 | |
| 检测限 | 1.62 mg/L | |
| 储存条件 | Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions | |
| 波长 | 340 nm | |
| 信号响应 | Increase | |
| 线性范围 | 1 to 50 μg of lactose (or 0.50 to 25 μg of D-glucose) | |
| 反应时间 | ~ 10 min | |
| 包装尺寸 | 10.00 in x 5.00 in x 2.00 in | |
| 包装重量 | 0.46 lb | |