Megazyme® Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
100 assays (manual) / 400 assays (auto-analyser)
SKU 编号 700004319 | 目录编号 K-MBG4
- Very cost effective
- All reagents stable for > 2 years
- Specific for endo-1,3:1,4-β-glucanase/lichenase
- Simple, convenient, rapid assay
- Well suited to automation
- Malt flour standard and lichenase standard included
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Content:
- (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) or
- (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
| 规格 | ||
|---|---|---|
| 品牌 | Megazyme | |
| 分析物 | β-Glucanase/Lichenase | |
| 平台 | 分光光度计, 自动分析仪 | |
| 结果类型 | 紫外吸光度 | |
| 批准 | Novel method | |
| 检测限 | (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL | |
| 储存条件 | Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions | |
| 波长 | 400 nm | |
| 应用实例 | Crude malt extracts, industrial enzyme preparations. | |
| 信号响应 | Increase | |
| 总检测时间 | Approx. 20 min (Malt β-glucanase); Approx. 10 min (Lichenase) | |
| 再现性 | ~ 3% | |
| 包装尺寸 | 10.00 in x 5.00 in x 2.00 in | |
| 包装重量 | 0.39 lb | |