Megazyme® Cellulase Assay Kit (CellG5 Method)
120 / 240 assays (manual) / 480 assays (auto-analyser)
SKU 编号 700004272 | 目录编号 K-CELLG5-4V
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for cellulase (endo-1,4-glucanase)
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Standard included
Cellulase Activity Assay Kit.
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.
| 规格 | ||
|---|---|---|
| 品牌 | Megazyme | |
| 分析物 | 内-纤维素酶 | |
| 平台 | 分光光度计, 自动分析仪 | |
| 结果类型 | 紫外吸光度 | |
| 批准 | Novel method | |
| 检测限 | 1.2 x 10-3 U/mL | |
| 储存条件 | Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions | |
| 波长 | 400 nm | |
| 应用实例 | Fermentation broths, industrial enzyme preparations and biofuels research. | |
| 信号响应 | Increase | |
| 总检测时间 | 10 min | |
| 再现性 | ~ 3% | |
| 包装尺寸 | 10.00 in x 5.00 in x 2.00 in | |
| 包装重量 | 0.44 lb | |