Megazyme® Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

SKU 编号  700004221  |  目录编号  E-HKGDH

数量:
最低数量要求:1
This item must be ordered in multiples of one case. Quantity per case: 1

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and analytical testing applications.

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规格
品牌Megazyme
EC 编号

Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49

储存条件

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

应用实例Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
配方In 3.2 M ammonium sulphate
外形Suspension
CAS 编号

Hexokinase: 9001-51-8
G6P-DH: 9001-40-5

浓度Hexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
同义词

Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

来源Yeast
表达Hexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
特异性

Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

单位定义

Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

最佳温度 (°C)40
包装尺寸0.80 in x 0.80 in x 1.90 in
包装重量0.06 lb