Synthetic Cannabinoids (UR‑144/XLR‑11) Forensic ELISA Kit
SKU 编号  133919  | 目录编号 
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Synthetic Cannabinoids (UR‑144/XLR‑11) Forensic ELISA Kit

SKU 编号  133919  |  目录编号 

  • Ready-to-use reagents
  • Highly sensitive
  • Multiple matrices
  • Large menu of tests available
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数量：
最低数量要求：20
This item must be ordered in multiples of one case. Quantity per case: 1

Neogen’s Synthetic Cannabinoids (UR-144/XLR-11) ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of XLR-11, UR-144, A-834735, and other synthetic cannabinoid metabolites. The kit was designed for screening purposes and is intended for forensic use only.

规格
品牌 Neogen®
分析物 UR-144 N-戊酸
应用 尿, 血, 口腔液, 取证矩阵
预期用途 For the determination of trace quantities of synthetic cannabinoids and metabolites in human blood, serum, and urine. Contact a Neogen representative for information on other matrices.
物种 人, 研究动物
Assay Sample Size 20 µL
平台 酶联免疫吸附
结果类型 定性
微孔板尺寸 96 well microplate
敏感性
Compound I-50 in EIA Buffer
UR-144 N-pentanoic acid 0.4ng/mL

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.
总检测孵育时间 75 minutes
药物面板 设计师药物, 致幻剂和麻醉剂
储存条件

2-8°C
波长
  • 650 nm with Red Stop Solution
  • 450 nm with acid stop
包装重量 0.70 lb
Compound % Cross-Reactivity
UR-144 N-pentanoic acid 100.00%
A-796260% 88.89%
UR-144 N-(5-hydroxypentyl) metabolite 88.89%
(±)-UR-144 N-(4-hydroxypentyl) metabolite 61.54%
XLR-11% 44.94%
A-834735% 36.36%
XLR11 N-(4-pentenyl) analog 20.00%
UR-144% 17.78%
UR-144 N-(2-hydroxypentyl) metabolite 10.53%
XLR-11 N-(4-hydroxypentyl) metabolite 6.56%
XLR11 Degradant 6.15%
UR-144 Degradant 2.11%
STS-135 (RM) 0.44%
AKB48 N-(5-fluoropentyl) metabolite 0.33%
AKB48% 0.18%
JWH-250 N-(5-hydroxypentyl) metabolite 0.10%
JWH-203% 0.08%
JWH-250 N-(5-carboxypentyl) metabolite 0.07%
JWH-250 N-(4-hydroxypentyl) metabolite 0.07%
JWH-250% 0.04%
AM 694% 0.03%
RCS-8% 0.02%
AM 1220% 0.02%
JWH-200% 0.01%
JWH-073% 0.01%
AM 2232% 0.01%
JWH-073 N-(4-hydroxybutyl) metabolite 0.01%
JWH-018 N-(5-hydroxypentyl) metabolite 0.01%
(+) JWH-018 N-(4-hydroxypentyl) metabolite < 0.04%
(±)-CP 47, 497 C7-hydroxy metabolite < 0.04%
AM 2201 N-(4-hydroxypentyl) metabolite < 0.04%
AM 2201% 0.004%
AB-PINACA < 0.004%
5-fluoro AB-PINACA < 0.004%
AM 2201 6-hydroxyindole metabolite < 0.004%
BB-22% < 0.004%
BB-22 3-carboxyindole metabolite < 0.004%
(-)-CP 47,497% < 0.004%
(±)-CP 47,497-C8 homolog < 0.004%
HU-210% < 0.004%
JWH-007% < 0.004%
JWH-015% < 0.004%
JWH-018% < 0.004%
JWH-018 4-hydroxyindole metabolite < 0.004%
JWH-018 5-hydroxyindole metabolite < 0.004%
JWH-018 6-hydroxyindole metabolite < 0.004%
JWH-018 N-(5-hydroxypentyl) β -D-glucuronide metabolite < 0.004%
JWH-018 N-pentanoic acid < 0.004%
JWH-019% < 0.004%
JWH-022% < 0.004%
JWH-073 N-butanoic acid metabolite < 0.004%
JWH-081% < 0.004%
JWH-122% < 0.004%
JWH-210% < 0.004%
JWH-250 5-hydroxyindole metabolite < 0.004%
JWH-398% < 0.004%
MAM2201% < 0.004%
1' Napthoyl Indole < 0.004%
PB-22% < 0.004%
5-fluoro PB-22% < 0.004%
PB-22 3-carboxyindole metabolite < 0.004%
PB-22 N-(4-hydroxypentyl) metabolite < 0.004%
PB-22 N-(5-hydroxypentyl) metabolite < 0.004%
PB-22 N-pentanoic acid < 0.004%
RCS-4% < 0.004%
RCS-4 N-(5-hydroxypentyl) metabolite < 0.004%
(+)WIN 55215-2 (mesylate) < 0.004%
  1. EIA Buffer
  2. Wash Buffer Concentrate (10X)
  3. K-Blue Substrate
  4. Drug-Enzyme Conjugate
  5. Antibody Coated Plate
  6. Red Stop Solution
  7. Qualitative QC Positive Control
  8. Qualitative QC Negative Control
  1. Deionized water.
  2. Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
  3. Graduated cylinder to dilute and mix wash buffer.
  4. Plate cover or plastic film to cover plate during incubation.
  5. Clean glassware (i.e. test tubes) to dilute samples.
  6. Microplate reader with 450 nm filter.

Optional Materials

  1. Microplate shaker.

科技

ELISA Assay Theory

Neogen’s direct competitive ELISAs operate on the basis of competition between the horseradish peroxidase (HRP) enzyme conjugate and the analyte in the sample for a limited number of specific binding sites on the precoated microplate.

ELISA Assay Procedure

Samples, standards or calibrators are first added to the precoated antibody microplate. Next, the enzyme conjugate is added and the mixture is incubated at room temperature. During incubation, competition for binding sites on the microplate is taking place. The plate is then washed, removing all unbound material. The bound enzyme conjugate is detected by the addition of a TMB-based substrate.

ELISA test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm or 450 nm if acid stop is used. The extent of color development is inversely proportional to the amount of analyte in the sample or standard.

ELISA Assay Test Principle Procedure

  1. Plates are precoated with the antibody. The plate is ready for use. DO NOT WASH.
  2. A sample or control is added to each well. Next the drug-enzyme conjugate is added and the mixture is incubated at room temperature.
  3. Wash the plate to remove all unbound materials.
  4. The bound materials now remain in the microplate.
  5. Add TMB substrate to each well and allow the color to develop.
  6. Qualitative results are obtained by measuring the absorbance reading at 650 nm or 450 nm if acid stop is used.
AssayPrincipleIllustration_Forensic.png

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LabLive 远程支持

The following are common examples of unexpected results when running Neogen's ELISA kits with possible explanations.

  1. Extreme deep blue color development with samples and standards.
    1. The plate was not properly washed (3 x 300 µL) with the diluted wash buffer. If using an automated washer, ensure the machine is working correctly and increase wash cycle to 5 x 300 µL.
    2. The enzyme conjugate concentrate was incorrectly diluted.

  2. Extremely low color development with samples and standards.
    1. The wash buffer was not diluted correctly before use.
    2. A poor quality of water was used to dilute the wash buffer. Deionized water should be used for dilutions.
    3. The enzyme conjugate concentrate was incorrectly diluted.
    4. The kit prematurely deteriorated, possibly from adverse shipping and storage conditions. Investigate the condition of the kit when it was received and how it was stored prior to use. Proper storage conditions are listed in the package insert.
    5. The kit has expired. Check the expiration dates on the test kit and reagents. No component of the kit should be used past the expiration date. Do not mix any reagents or components of one kit with the reagents or components of another kit.
    6. Contamination. Always use aseptic techniques when opening and removing reagents from vials and bottles. Keep the plate covered except when adding reagents, washing or reading. Always use different pipette tips for each reagent. When pipetting, do not allow the pipette tip to touch any of the reagents already in the well.

  3. No color development with samples and standards.
    1. Improper dilution of enzyme conjugate concentrate.
    2. The kit has expired. Check the expiration dates on the test kits and reagents. No components of the kit should be used past the expiration date. Do not mix any reagents or components of one kit with the reagents or components of another kit.
    3. Incorrect addition of kit reagents. Review assay procedure.

  4. Little to no displacement with the standard curve.
    1. Incorrect dilution of standards. Refer to the dilution scheme in the package insert.
    2. Contamination.
    3. The plate was not properly washed (3 x 300 µL) with the diluted wash buffer. If using an automated washer, ensure the machine is working correctly and increase wash cycle to 5 x 300 µL.
    4. Standard has deteriorated prematurely. Contact your Neogen representative and provide them with the kit name, lot number, expiration date and your OD readings for further investigation.

  5. The standard curve performed correctly, but the known negative samples gave low color development.
    1. Samples need to be diluted or extracted to eliminate interference. Refer to the extraction procedure in the kit insert.

  6. The standard curve performed correctly, but the extracted samples gave low color development.
    1. The concentration of the analyte in the extracted sample is too high. The extracted sample will need to be diluted before running it in the assay so the sample OD reading will fit in the standard curve. When a dilution is used, the concentration determined from the standard curve must be multiplied by the dilution factor.
    2. Inadequate extraction of samples resulting in the presence of a solvent. Refer to the recommended extraction procedure in the package insert.

  7. Variability with duplicates.
    1. Inconsistent and/or inadequate pipetting technique when adding reagents. Improve pipetting technique.
    2. Inconsistent washing. The wells should be washed 3 x 300 µL with the diluted wash buffer. If using an automated washer, ensure the machine is working correctly and increase wash cycle to 5 x 300 µL.
    3. Inadequate aspiration during washing. The wells should be dumped and tapped between each washing. Tap out excess liquid in wells before adding substrate. Add substrate immediately to the wells once the excess wash buffer has been removed. If using an automated washer, ensure the machine is aspirating correctly.
    4. Interruption during assay set-up. Have all reagents prepared before assay set-up commences. Reagent addition should be performed in a timely and accurate manner.