Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)
SKU Nr.  700004214  | Katalognummer  E-FRLQPU
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Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)

10 ml / 20,000 Units

SKU Nr.  700004214  |  Katalognummer  E-FRLQPU

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To order or for more information contact our food safety team
To order or for more information contact our food safety team

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Components: exo-Inulinase (2,000 U/mL on kestose, at 40°C) and endo-Inulinase (100 U/mL on inulin at 40°C).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

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Spezifikationen
MarkeMegazyme
EC-Nummer

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Lagerbedingungen

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

AnwendungsbeispieleApplications in carbohydrate research and in the food industry.
FormulierungIn 50% (v/v) glycerol plus 0.02% sodium azide
Physische FormSolution
CAS-Nummer

9001-57-4,
9025-67-6,
37288-56-5

Konzentrationendo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 10,000 U
Synonyme

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

QuelleAspergillus niger
AusdruckRecombinant from Aspergillus niger
Spezifität

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Einheitendefinition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Temperaturoptima (°C)60
Abmessungen der Packung0,80 Zoll x 0,80 Zoll x 1,90 Zoll
Packungsgewicht0,06 lb