Megazyme® Fructanase Mixture (Ultrapure, recombinant, liquid)
10 ml / 20,000 Units
SKU 编号 700004214 | 目录编号 E-FRLQPU
High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.
For Fructan Determination.
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.
Components: exo-Inulinase (2,000 U/mL on kestose, at 40°C) and endo-Inulinase (100 U/mL on inulin at 40°C).
Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
| 规格 | ||
|---|---|---|
| 品牌 | Megazyme | |
| EC 编号 | endo-Inulinase: 3.2.1.7, | |
| 储存条件 | Storage Temperature: Below -10°C Stability: > 1 year under recommended storage conditions. Check vial for details | |
| 应用实例 | Applications in carbohydrate research and in the food industry. | |
| 配方 | In 50% (v/v) glycerol plus 0.02% sodium azide | |
| 外形 | Solution | |
| CAS 编号 | 9001-57-4, | |
| 浓度 | endo-inulinase: Supplied at ~ 1,000 U exo-inulinase: Supplied at ~ 10,000 U | |
| 同义词 | endo-inulinase: 1-beta-D-fructan fructanohydrolase | |
| 来源 | Aspergillus niger | |
| 表达 | Recombinant from Aspergillus niger | |
| 特异性 | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. | |
| 单位定义 | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. | |
| 最佳温度 (°C) | 60 | |
| 包装尺寸 | 0.80 in x 0.80 in x 1.90 in | |
| 包装重量 | 0.06 lb | |