Megazyme® Fructanase Mixture (powder)
SKU 编号  700004216  | 目录编号  E-FRMXPD
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Megazyme® Fructanase Mixture (powder)

20,000 Units

SKU 编号  700004216  |  目录编号  E-FRMXPD

AOAC OMA

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数量：
最低数量要求：1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact our food safety team
To order or for more information contact our food safety team

High purity Fructanase Mixture (powder) for use in research, biochemical enzyme assays and analytical testing applications.

Components: exo-Inulinase 20,000 U (on kestose at 40°C), endo-inulinase 1000 U (on fructan at 40°C).

Fructanase, along with Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY), can also be utilised in the enzyme hydrolysis step for determining polydextrose a low molecular weight dietary fiber in food samples.

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan and Polydextrose.

Megazyme_Scheme-E-FRMXPD.png
规格
品牌Megazyme
批准

For use with AOAC OMA 997.08, 2000.11, 2016.06

EC 编号

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

储存条件

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

配方Supplied as a lyophilised powder
外形Powder
CAS 编号

9001-57-4,
9025-67-6,
37288-56-5

同义词

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

来源Aspergillus sp.
表达From Aspergillus sp.
特异性

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

单位定义

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

最佳温度 (°C)40
包装尺寸0.80 in x 0.80 in x 1.90 in
包装重量0.03 lb