Alert® for Total Milk
SKU 编号  700002579  | 目录编号  8471
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Alert® for Total Milk

SKU 编号  700002579  |  目录编号  8471

  • Minimal equipment required
  • Screen single or multiple samples at one time
  • Test food, rinses or swabs
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数量：
最低数量要求：1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact us
To order or for more information contact us

Alert® for Total Milk Allergen is used as a screening tool to test raw ingredients, in clean-in-place rinses, on environmental surfaces, or product throughout the production process, for the presence of milk residue.

规格
品牌Alert®
分析物牛奶
平台酶联免疫吸附
结果类型定性
检测限

5 ppm, 10 ppm NFDM

测试时间30.00
Prop 65（65 号提案）警告
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
每个包裹的数量20 tests
包装尺寸10.50 in x 8.00 in x 3.00 in
包装重量0.60 lb
  1. 24 antibody-coated microwells
  2. 2 yellow-labeled bottles of controls made from nonfat dried milk; 1 bottle at a concentration of 5 ppm and 1 bottle at 10 ppm (see procedural note No. 1)
  3. 1 blue-labeled bottle of enzyme-labeled antibody conjugate
  4. 1 green-labeled dropper bottle of K-Blue® Substrate solution
  5. 1 red-labeled dropper bottle of Red Stop solution
  6. 20 unlabeled empty sample dropper bottles with tips
  7. 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  8. 1 wide-mouth bottle of 40 mL PBS-Tween wash buffer concentrate; a bottle contains enough concentrate to prepare 1 L of wash buffer
  9. 50 g of extraction additive in a specimen cup
  10. Plastic scoop to measure extraction additive
  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile swabs
    2. 100 disposable dropper tips
  3. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)
  4. Shaker water bath, hot plate or equivalent heat source capable of maintaining 60 ± 1°C
  5. Thermometer
  6. Two 1 L bottles to prepare washing and extraction solutions (Neogen item 9472)
  7. Paper towels or equivalent absorbent material
  8. Microwell holder (Neogen item 9402)
  9. Waterproof marker
  10. Wash bottle (Neogen item 9400)
  11. Distilled or deionized water
  12. Timer (Neogen item 9426)
  13. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  14. Pipettor, 100 µL (Neogen item 9272, 9278, 9276)
  15. Pipette tips (Neogen item 9410, 9407, 9417)
  16. Vortex (Neogen item 9494)
  17. 50 mL sample tubes (Neogen item 9381)

科技

First, samples and controls are added to their respective wells. During an incubation, the target substance binds to the antibodies. The wells are washed to remove all unbound materials.

An enzyme conjugate (antibody chemically linked with an enzyme) is added to all wells. During an incubation, conjugate binds to the already bound target substance, forming a “sandwich.” The more target substance in the wells, the more conjugate that binds in the wells.

The wells are washed again to remove all unbound conjugate.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, color develops in proportion to the amount of conjugate in the wells. The more conjugate, the more color, and the more target substance that is detected.

Results are read visually in a screening format—the more blue color, the more target substance detected.

Alert_diagram.png

培训

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

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