Reveal® 2.0 for PSP (paralytic shellfish poisoning) is a 5 minute lateral flow assay that detects saxitoxin equivalents. Paralytic Shellfish Poisoning causing toxins can be produced by dinoflagellates of different genera including Alexandrium and Gymnodinium. Once the toxin is present in the shellfish tissue it cannot be destroyed by heat during cooking. Reveal 2.0 for PSP is intended for the qualitative screening of shellfish PST’s, which produce a positive result with samples containing approximately 800 ppb STX equivalents or greater.
Specifications
Analyte PSP
Brand Reveal®
Limit of Detection 800 ppb STX
Matrix Shellfish
Package Dimensions 8.60 IN x 7.80 IN x 3.30 IN
Package Weight 2.00 LB
Platform Lateral Flow
Quantity per Package 24 tests
Result Type Qualitative
Testing Time 5.00 Minutes
Unit Analyte PSP

  1. 24 Reveal 2.0 for PSP lateral flow test strips
  2. 24 microwells
  3. 24 vials of PSP buffer
  4. 25 extraction bags
  5. 48 disposable 100 µL pipettors
  1. Marine Biotoxins Starter Kit (Neogen® item 9563)
    1. Microwell holder
    2. 1 roller
    3. 1 bag clip (white clip and green straw)
  2. 2. Distilled water
  3. Sample collection cups with lids (Neogen items 9428, 9428B)
  4. Blender (Neogen items 9493, 9477 or 9495)
  5. Scale capable of weighing 0.5–400 g ± 0.1 g (Neogen item 9427)
  6. Timer (Neogen item 9452)
  7. Graduated cylinder, 50 mL (Neogen item 9447) or bottle-top dispenser (Neogen item 9448)
  8. AccuScan Pro reader (Neogen item 9565)

Additional Information

For use with AccuScan Pro.

Reveal 2.0 for PSP is a single-step lateral flow device based on a competitive immunoassay format. Shellfish extract is wicked through a reagent zone containing antibodies specific for PSTs that have been conjugated to colored particles. If PSTs are present in the sample, the toxin will be captured by the particle-antibody complex. The complex then is wicked onto a membrane, which contains a stationary capture zone of a toxin-protein conjugate. This zone captures any uncomplexed PST particle-antibody. Therefore, as the concentration of PSTs in the sample increases, the test line intensity decreases. The membrane also contains a stationary control zone which will always form regardless of the level of PSTs.

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