Reveal® 2.0 for DSP (diarrhetic shellfish poisoning) is a single step lateral flow assay that detects okadaic acid (OA) and dinophysistoxin (DTX) derivatives. These marine biotoxins can accumulate in shellfish during toxic algal blooms. Once the toxin is present in the shellfish tissue it cannot be destroyed by heat during cooking. The E.U. and U.S. have established maximum threshold levels of 160 ppb.
Specifications
Analyte DSP
Brand Reveal®
Limit of Detection 160 ppb
Matrix Shellfish
Package Dimensions 8.40 IN x 5.40 IN x 2.25 IN
Package Weight 0.80 LB
Platform Lateral Flow
Quantity per Package 24 tests
Result Type Qualitative
Testing Time 15.00 Minutes
Unit Analyte DSP

  1. 24 Reveal 2.0 for DSP lateral flow test strips
  2. 24 wells
  3. 24 vials of DSP buffer A (clear cap)
  4. 24 vials of DSP buffer B (red cap)
  5. 25 extraction bags
  6. 48 disposable 100 µL pipettors
  1. Marine Biotoxins Starter Kit (Neogen® item 9563)
    1. Microwell holder
    2. 1 roller
    3. 1 bag clip (white clip and green straw)
  2. DSP Hydrolysis Pack (Neogen item 9554)
    1. 2.5 M sodium hydroxide (NaOH) solution (5 mL)
    2. 2.5 M hydrochloric acid (HCl) solution (5 mL)
    3. 24 glass sample vials
  3. Distilled water
  4. Methanol (analytical grade recommended, VWR 20864.320)
  5. Filter syringes (Neogen item 9420)
  6. Sample collection cups with lids (Neogen items 9428, 9428B)
  7. Blender (Neogen items 9493, 9477 or 9495)
  8. Scale capable of weighing 0.5–400 g ± 0.1 g (Neogen item 9427)
  9. Timer (Neogen item 9452)
  10. Graduated cylinder, 50 mL (Neogen item 9447)
  11. Sample collection tubes with caps, 5 mL (Neogen item 9421, 9421B)
  12. Pipettor, 100–1000 mL, and pipette tips (Neogen item 9463, 9464)
  13. Heater block (capable of holding 76 ± 2°C (Recommended VWR 460-3250 & 460-3280)
  14. AccuScan Pro reader (Neogen item 9565)

Additional Information

For use with AccuScan Pro.

Reveal 2.0 for DSP is a single-step lateral flow device based on a competitive immunoassay format. In summary, the shellfish extract is wicked through a reagent zone, containing antibodies specific for OA-group toxins that have been conjugated to colored particles. If the toxins are present in the sample, the toxin will be captured by the particle-antibody complex. The complex then is wicked onto a membrane, which contains a stationary capture zone of a toxin-protein conjugate. This zone captures any uncomplexed toxin particle-antibody. Therefore, as the concentration of toxins in the sample increases, the test line intensity decreases. The membrane also contains a stationary control zone which always will form regardless of the level of toxins.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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