Reveal® for 2.0 ASP (amnesic shellfish poisoning) is a 10 minute lateral flow assay that detect domoic acid. Domoic acid is a marine biotoxin that is secreted by algal blooms and may be found in shellfish. Once the toxin is present in the shellfish tissue it cannot be destroyed by heat during cooking. Reveal 2.0 for ASP detect domoic acid at the established U.S. and E.U. maximum threshold of 20 ppm.
Specifications
Analyte ASP
Brand Reveal®
Cross Reactivity Domoic Acid
Limit of Detection 20 ppm
Package Dimensions 8.30 IN x 5.20 IN x 2.40 IN
Package Weight 0.70 LB
Platform Lateral Flow
Quantity per Package 24 tests
Result Type Qualitative
Testing Time 10.00 Minutes

  1. 24 Reveal 2.0 for ASP lateral flow test strips
  2. 24 microwells
  3. 24 vials of ASP buffer
  4. 25 extraction bags
  5. 48 disposable 100 µL pipettors
  1. Marine biotoxins starter kit (Neogen® item 9563)
    1. Microwell holder
    2. 1 roller
    3. 1 bag clip (white clip and green straw)
  2. Distilled water
  3. Sample collection cups with lids (Neogen items 9428, 9428B)
  4. Blender (Neogen items 9493, 9477 or 9495)
  5. Scale capable of weighing 0.5–400 g ± 0.1 g (Neogen item 9427)
  6. Timer (Neogen item 9452)
  7. Graduated cylinder, 50 mL (Neogen item 9367) or bottle-top dispenser (Neogen item 9448)
  8. AccuScan Pro reader (Neogen item 9565)

Additional Information

For use with AccuScan Pro.

Reveal 2.0 for ASP is a single-step lateral flow device based on a competitive immunoassay format. Shellfish extract is wicked through a reagent zone containing antibodies specific for DA which have been conjugated to colored particles. If DA is present in the sample, the toxin will be captured by the particle-antibody complex. The complex then is wicked onto a membrane, which contains a stationary capture zone of a toxin-protein conjugate. This zone captures any uncomplexed DA particle-antibody. Therefore, as the concentration of DA in the sample increases, the test line intensity decreases. The membrane also contains a stationary control zone which will always form regardless of the level of DA.

Training

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