Alert® for Histamine is a competitive direct ELISA intended for the screening of histamine in scombroid species of fish, such as tuna, bluefish and mahi-mahi, and in fishmeal.
Specifications
Analyte Histamine
Brand Alert®
Cross Reactivity Specific for histamine. No cross-reactivity with other biogenic amines.
Limit of Detection 50 ppm
Package Dimensions 10.50 IN x 8.00 IN x 3.00 IN
Package Weight 1.30 LB
Platform ELISA
Quantity per Package 20 tests
Result Type Qualitative
Testing Time 20.00 Minutes

  1. 24 antibody-coated microwells
  2. 24 red-marked mixing wells
  3. 1 yellow-labeled bottle of 50 ppm histamine control
  4. 1 blue-labeled bottle of histamine-HRP conjugate solution
  5. 20 bottles of sample extract diluent buffer
  6. 1 bottle of 80 mL wash buffer
  7. 1 green-labeled bottle of K-Blue® Substrate solution
  8. 1 red-labeled bottle of Red Stop solution
  9. 12 disposable beakers
  1. Alert for Histamine extraction kit (Neogen item® 9520) — contains 20 bottles, 20 filter syringes and 20 tubes
  2. Filter syringes for histamine (Neogen item 9420H), Whatman no. 1 filter paper (Neogen item 9430), centrifuge or equivalent
  3. Tubes with caps, 5 mL (Neogen items 9421, 9421B)
  4. Graduated cylinder
  5. Blender
  6. Scale capable of weighing 10-50 grams (Neogen item 9427)
  7. Pipettor, 100 µL (Neogen items 9272, 9290)
  8. Pipette tips (Neogen items 9410, 9407, 9417)
  9. Paper towels or equivalent absorbent material
  10. Microwell holder (Neogen item 9402)
  11. Timer (Neogen item 9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item 9400)
  14. Distilled or deionized water

First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.

After an incubation, the wells are washed to remove all unbound materials.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.

Results are read visually in a screening format—the less blue color, or more red, the more target substance detected. In a quantitative format, results are obtained by measuring the wells’ color change in a microwell reader and comparing the readings against a standard curve.

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Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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Validation

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