Reveal® Q+ for Ochratoxin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format intended for the quantitative testing of ochratoxin in grain and grain products.
Specifications
Analyte Ochratoxin
Brand Reveal®
Limit of Detection 2 ppb
Package Dimensions 8.40 IN x 5.20 IN x 2.50 IN
Package Weight 0.55 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 2.00 ppb - 20.00 ppb
Result Type Quantitative
Testing Time 9.00 Minutes

  1. 25 Reveal Q+ for Ochratoxin test strips
  2. 25 red sample dilution cups
  3. 25 sample cups
  4. 1 bottle of sample diluent
  5. Instructions for use
  1. 70% methanol solution (Neogen® item 8055, 8056)
  2. Sample collection cups with lids (Neogen item 9428)
  3. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  4. Scale capable of weighing 5–50 g ± 0.1 g (Neogen item 9427)
  5. Timer (Neogen item 9426)
  6. Reveal sample cup rack (Neogen item 9475)
  7. AccuScan reader: AccuScan III (Neogen item 9590), AccuScan Pro (Neogen item 9565), or AccuScan Gold (Neogen item 9595)
  8. Dispensing pump or graduated cylinder (Neogen item 9448, 9447)
  9. Filter syringe (Neogen item 9420)
  10. Sample collection tubes with caps (Neogen item 9421, 9421B)
  11. Pipettor, 100 µL (Neogen item 9272, 9278)
  12. Pipette tips, 100 µL (Neogen item 9407, 9410, 9417)
  13. Pipettor, 200 µL (Neogen item 9488)
  14. Pipette tips, 200–1000 µL (Neogen item 9464, 9487)

Assay Principle

Reveal Q+ for Ochratoxin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for ochratoxin conjugated to colloidal gold particles. If ochratoxin is present, it will be captured by the particle-antibody complex. The ochratoxin-antibody-particle complex then is wicked onto a membrane, which contains a zone of ochratoxin conjugated to a protein carrier. This zone captures any uncomplexed ochratoxin antibody, allowing the particles to concentrate and form a visible line. As the level of ochratoxin in a sample increases, free ochratoxin will complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of ochratoxin in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® reader convert these line densities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of ochratoxin, ensuring the strip is functioning properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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Validation

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