Agri-Screen® for Fumonisin

Item No.  8810

  • Rapid and easy onsite testing
  • Screen at various levels
  • No reader required
  • Broad range of validated and approved commodities
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Minimum quantity required: 0
Agri-Screen® for Fumonisin is a competitive direct ELISA that provides a visible screening result against a known control of fumonisin in such commodities as barley, corn, DDGS, milo, popcorn, rice, soybeans and wheat.
Analyte Fumonisin
Brand Agri-Screen®
Cross Reactivity Total fumonisins (B1, B2, B3)
Limit of Detection 5 ppm
Package Dimensions 10.50 IN x 8.00 IN x 3.50 IN
Package Weight 0.70 LB
Prop 65 Warning
WARNING: This product can expose you to chemicals including fumonisin B1, which is known to the State of California to cause cancer, and thimerosal and methanol, which are known to the State of California to cause birth defects or other reproductive harm. For more information go to
Quantity per Package 12 tests
Result Type Qualitative
Testing Time 15.00 Minutes

  1. 24 antibody-coated microwells
  2. 24 red-marked mixing wells
  3. 1 yellow-labeled bottle of 5 ppm fumonisin control (see precautions for handling of methanol solution)
  4. 1 blue-labeled bottle of fumonisin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. 14 pink-labeled bottles for sample dilution, prefilled with 7.9 mL of deionized water (Dilution bottles not provided with Neogen item #8811)
  8. Directions for use
  1. Extraction materials (items 4 through 6 available in kit form from Neogen, item #8052):
    1. 70% methanol solution (Neogen item #8055)
    2. Distilled or deionized water
    3. 250 mL graduated cylinder (Neogen item #9368)
    4. Container with 125 mL capacity (Neogen item #9428)
    5. Neogen filter syringes, Whatman #1 filter paper, or equivalent (Neogen item #9420/#9430)
    6. Sample collection tubes (Neogen item #9421)
  2. 50 mL graduated cylinder (Neogen item #9447)
  3. High-speed blender (Neogen item #9493/#9477)
  4. Agri-Grind grinder or equivalent (Neogen item #9401/#9453)
  5. Scale capable of weighing 5-25 grams (Neogen item #9427)
  6. Pipettor 100 µL (Neogen item #9272/#9278)
  7. Tips for 100 µL pipettor (Neogen item #9410/#9407)
  8. Paper towels or equivalent absorbent material


First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.

After an incubation, the wells are washed to remove all unbound materials.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.

Results are read visually in a screening format—the less blue color, or more red, the more target substance detected.



Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more