Agri-Screen® for DON

Item No.  8310

  • Rapid and easy onsite testing
  • Screen at various levels
  • No reader required
  • Broad range of validated and approved commodities
$185.50
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Out of stock
Expect availability in 2-4 weeks
Agri-Screen® for DON is used for the screening analysis of DON in grain and grain products, such as wheat, wheat midds, wheat flour, wheat bran, barley, corn, cornmeal, corn screenings, malted barley and oats.
Specifications
Analyte Deoxynivalenol (DON)
Brand Agri-Screen®
Cross Reactivity DON 100%. Also cross-reactive with 3-acetyl DON but does not bind to other trichothecenes.
Limit of Detection 1 ppm
Package Dimensions 8.14 IN x 5.04 IN x 1.01 IN
Package Weight 0.10 LB
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including pyridine, which is known in the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 12 tests
Result Type Qualitative
Testing Time 10.00 Minutes

  1. 24 antibody-coated microwells
  2. 24 red-marked mixing wells
  3. 1 yellow-labeled bottle of 1 ppm DON control
  4. 1 blue-labeled bottle of DON-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. Directions for use
  1. Extraction materials (items 2 through 4 available in kit form from Neogen, item #8052):
    1. Distilled or deionized water
    2. Sample containers with 125 mL capacity (Neogen item #9428)
    3. Neogen filter syringe, Whatman #1 filter paper, or equivalent (Neogen item #9420/#9430)
    4. Sample collection tubes (Neogen item #9421)
  2. 50 mL graduated cylinder (Neogen item #9447)
  3. Agri-Grind grinder or equivalent (Neogen item #9401)
  4. Scale capable of weighing 5 – 25 grams (Neogen item #9427)
  5. 100 µL pipettor (Neogen item #9272/#9278)
  6. Tips for 100 µL pipettor (Neogen item #9410/#9407)
  7. Paper towels or equivalent absorbent material
  8. Plastic bucket for use as a waste receptacle
  9. Microwell holder (Neogen item #9402)
  10. Timer (Neogen item #9426)
  11. Waterproof marker
  12. Wash bottle (Neogen item #9400)
  13. Distilled or deionized water

First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.

After an incubation, the wells are washed to remove all unbound materials.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.

Results are read visually in a screening format—the less blue color, or more red, the more target substance detected.

Agri-Screen_diagram.png

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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