Agri-Screen® for Aflatoxin is a competitive direct ELISA that provides a visible screening result against a known control of aflatoxin in commodities such as corn, cornmeal, corn gluten meal, corn/soy blend, wheat, rice, milo, soy, whole cottonseed, cottonseed meal, raw peanuts, peanut butter and mixed feeds.
Specifications
Analyte Aflatoxin
Approvals AOAC Official Method 990.32
Brand Agri-Screen®
Cross Reactivity Total aflatoxins (B1, B2, G1, G2)
Limit of Detection 20 ppb
Package Dimensions 8.80 IN x 1.60 IN x 0.80 IN
Package Weight 0.20 LB
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including methanol, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 12 tests
Result Type Qualitative
Testing Time 5.00 Minutes

  1. 24 antibody-coated microwells
  2. 24 red-marked mixing wells
  3. 1 yellow-labeled bottle of 20 ppb aflatoxin control (see precautions for handling of methanol solution)
  4. 1 blue-labeled bottle of aflatoxin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  1. Extraction materials (items b through d available in kit form from Neogen, item #8052):
    1. 70% ACS methanol solution (Neogen item #8055/8056)
    2. Container with 125 mL capacity (Neogen item #9428)
    3. Neogen filter syringes, Whatman #1 filter paper, or equivalent (Neogen item #9420/9430)
    4. Sample collection tubes (Neogen item #9421)
  2. Graduated cylinder, 50 mL (Neogen item #9447)
  3. Blender, high-speed (Neogen item #9493)
  4. Agri-Grind grinder or equivalent (Neogen item #9401)
  5. Scale capable of weighing 5-50 grams (Neogen item #9427)
  6. Pipettor, 100 µL (Neogen item #9272 or 9278)
  7. Pipettor tips for 100 µL pipettor (Neogen #9410)
  8. Paper towels or equivalent absorbent material
  9. Plastic bucket for use as waste receptacle
  10. Microwell holder (Neogen item #9402)
  11. Timer (Neogen item #9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item #9400)
  14. Distilled or deionized water

Agri-Screen® for Aflatoxin, Bahasa Malaysia - Malaysia Agri-Screen® for Aflatoxin, Chinese Simplified - China Agri-Screen® for Aflatoxin, Chinese Traditional - Taiwan Agri-Screen® for Aflatoxin, Danish - Denmark Agri-Screen® for Aflatoxin, Dutch Agri-Screen® for Aflatoxin, English - Australia Agri-Screen® for Aflatoxin, English - Canada Agri-Screen® for Aflatoxin, English - Great Britain Agri-Screen® for Aflatoxin, English - New Zealand Agri-Screen® for Aflatoxin, English - US Agri-Screen® for Aflatoxin, English - US Agri-Screen® for Aflatoxin, Finnish - Finland Agri-Screen® for Aflatoxin, French Agri-Screen® for Aflatoxin, French - Canada Agri-Screen® for Aflatoxin, German Agri-Screen® for Aflatoxin, Hungarian - Hungary Agri-Screen® for Aflatoxin, Indonesian - Indonesia Agri-Screen® for Aflatoxin, Italian - Italy Agri-Screen® for Aflatoxin, Japanese - Japan Agri-Screen® for Aflatoxin, Korean - Korea Agri-Screen® for Aflatoxin, Portuguese - Brazil Agri-Screen® for Aflatoxin, Spanish - Mexico/Central America Agri-Screen® for Aflatoxin, Spanish - South America Agri-Screen® for Aflatoxin, Swedish - Sweden Agri-Screen® for Aflatoxin, Danish - Denmark Agri-Screen® for Aflatoxin, English - Great Britain Agri-Screen® for Aflatoxin, French Agri-Screen® for Aflatoxin, German Agri-Screen® for Aflatoxin, Hungarian - Hungary Agri-Screen® for Aflatoxin, Spanish - Mexico/Central America Agri-Screen® for Aflatoxin, Swedish - Sweden

First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.

After an incubation, the wells are washed to remove all unbound materials.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.

Results are read visually in a screening format—the less blue color, or more red, the more target substance detected.

Agri-Screen_diagram.png

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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Validation

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