Yersinia Slective Agar (Schiemann's CIN Agar)

Item No.  NCM0182A

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Yersinia Selective Agar is used with cefsulodin and novobiocin for the isolation of Yersinia enterocolitica in a laboratory setting. Yersinia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This medium is based on the work of Schiemann. It is used for the isolation and enumeration of Yersinia spp. from clinical samples and from food. The selective components are sodium desoxycholate, crystal violet, cefsulodin, irgasan and novobiocin. Yersiniae ferment mannitol with an intense, localized, acid production in the center of the colony which produces a red ‘bull’s eye’ appearance. The ratio of transparent border to red centre varies with serotype and environmental strains may appear rough with an irregular edge. Most other enteric bacteria, if they grow, produce a larger colony with a diffuse pinkish center and opaque outer zone.

Specifications
Analyte Yersinia spp.
Brand NEOGEN Culture Media
Formula
Formula Liter
Peptone Mix 22.5 g/L
Mannitol 20.0 g/L
Sodium Chloride 1.0 g/L
Magnesium Sulphate 0.1 g/L
Sodium Pyruvate 2.0 g/L
Sodium Deoxycholate 0.5 g/L
Neutral red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 12.0 g/L
Grams per Liter 58.00
Liters per 500g 9.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 470.00
Preparation
  1. Suspend 19.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.
Previous Item Number Product was previously known as Acumedia Product: 7257 Yersinia Selective Agar; and LabM Product: LAB120 Yersinia C.I.N. Agar Base

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