Wilkins-Chalgren Broth

Item No.  NCM0219A

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Wilkins-Chalgren Broth is used for the cultivation of anaerobic microorganisms in a laboratory setting. Wilkins-Chalgren Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Wilkins-Chalgren Broth is derived from Wilkins-Chalgren Agar. Wilkins-Chalgren Broth provides good growth of anaerobes. This medium was selected because it does not require the addition of blood to support satisfactory growth of most anaerobes. Wilkins-Chalgren Broth is the same formula as Wilkins-Chalgren Agar with agar omitted.

The survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature.

Analyte Anaerobic
Brand NEOGEN Culture Media
Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Enzymatic Digest of Animal Tissue 10.0 g/L
Yeast Extract 5.0 g/L
Sodium Chloride 5.0 g/L
Dextrose 1.0 g/L
L-Arginine 1.0 g/L
Sodium Pyruvate 1.0 g/L
Hemin 0.005 g/L
Vitamin K 0.0005 g/L
Grams per Liter 33.00
Liters per 500g 15.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 33 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7233 Wilkins-Chalgren Broth



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